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鉴定和描述水稻钾离子通道基因家族及其对盐和冷胁迫的响应。

Identification and Characterization of Potassium Channel Gene Family and Response to Salt and Chilling Stress in Rice.

机构信息

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.

Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 311121, China.

出版信息

Int J Mol Sci. 2024 Sep 8;25(17):9728. doi: 10.3390/ijms25179728.

Abstract

potassium channel proteins are a class of voltage-gated ion channels responsible for K uptake and translocation, playing a crucial role in plant growth and salt tolerance. In this study, bioinformatic analysis was performed to identify the members within the gene family. Moreover, the expression patterns of rice K channel genes were analyzed in different tissues and salt treatment by RT-qPCR. The results revealed that there were eight K channel genes distributed on chromosomes 1, 2, 5, 6 and 7 in rice, and their promoters contained a variety of cis-regulatory elements, including hormone-responsive, light-responsive, and stress-responsive elements, etc. Most of the K channel genes were expressed in all tissues of rice, but at different levels in different tissues. In addition, the expression of K channel genes differed in the timing, organization and intensity of response to salt and chilling stress. In conclusion, our findings provide a reference for the understanding of K channel genes, as well as their potential functions in response to salt and chilling stress in rice.

摘要

钾通道蛋白是一类电压门控离子通道,负责 K 的摄取和转运,在植物生长和耐盐性中起着至关重要的作用。在这项研究中,进行了生物信息学分析以鉴定基因家族中的成员。此外,通过 RT-qPCR 分析了不同组织和盐处理中水稻 K 通道基因的表达模式。结果表明,在水稻的 1、2、5、6 和 7 号染色体上分布着 8 个 K 通道基因,它们的启动子包含多种顺式调控元件,包括激素响应、光响应和应激响应元件等。大多数 K 通道基因在水稻的所有组织中表达,但在不同组织中的表达水平不同。此外,K 通道基因对盐和冷胁迫的响应在时间、组织和强度上存在差异。总之,我们的研究结果为理解 K 通道基因及其在水稻响应盐和冷胁迫中的潜在功能提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe76/11395327/371741f795a3/ijms-25-09728-g001.jpg

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