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用一步孔雀绿法测定血清和尿液中的磷酸盐。

Determination of phosphate in serum and urine by a single step malachite-green method.

作者信息

Kallner A

出版信息

Clin Chim Acta. 1975 Feb 22;59(1):35-9. doi: 10.1016/0009-8981(75)90215-6.

DOI:10.1016/0009-8981(75)90215-6
PMID:1122646
Abstract

The highly sensitive malachite-green method for the determination of phosphate requires a protective colloid in order to avoid precipitation of the formed dye salt. A polyvinylalcohol proved to be suitable and permit the determination of phosphate in urine and in serum without prior precipitation of the proteins. The present method is suitable for a one-step procedure which allows the analysis of several hundred samples per man-hour and is applicable to microquantities of serum or urine.

摘要

用于测定磷酸盐的高灵敏度孔雀石绿方法需要一种保护胶体，以避免所形成的染料盐沉淀。已证明聚乙烯醇是合适的，它使得无需预先沉淀蛋白质就能测定尿液和血清中的磷酸盐。本方法适用于一步程序，每工时可分析数百个样品，并且适用于微量的血清或尿液。

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