Holtkamp H C, Verhoef N J, Leijnse B
Clin Chim Acta. 1975 Feb 22;59(1):41-9. doi: 10.1016/0009-8981(75)90216-8.
The glucose concentrations in whole blood (WB) and plasma (P), both prediluted with distilled water and saline, and in their protein-free filtrates, were determined with the Hoffman ferricyanide method. In whole blood prediluted with distilled water the glucose concentration appeared to be 13% higher than in whole blood prediluted with saline. In plasma, predilution with distilled water or saline did not result in different glucose values. Plasma glucose is significantly higher than whole blood glucose independent of the method of pretreatment. A direct relationship between the two values exists, which for protein-free filtrates is represented by Glu(P) equals 1.07 Glu(WB) + 0.11. The glucose concentrations in erythrocytes were calculated and also correlated to plasma glucose values. A good correlation was found when protein-free filtrates were used. Apparently, conversion factors from whole blood to plasma glucose may be used only in cases where samples are deproteinized.
采用霍夫曼铁氰化物法测定了用蒸馏水和生理盐水预稀释的全血（WB）和血浆（P）及其无蛋白滤液中的葡萄糖浓度。在以蒸馏水预稀释的全血中，葡萄糖浓度似乎比以生理盐水预稀释的全血高13%。在血浆中，用蒸馏水或生理盐水预稀释不会导致葡萄糖值出现差异。无论预处理方法如何，血浆葡萄糖均显著高于全血葡萄糖。两者之间存在直接关系，对于无蛋白滤液，这种关系表现为Glu(P) = 1.07 Glu(WB) + 0.11。计算了红细胞中的葡萄糖浓度，并将其与血浆葡萄糖值进行了关联。当使用无蛋白滤液时，发现两者具有良好的相关性。显然，只有在样品进行了脱蛋白处理的情况下，才可以使用从全血到血浆葡萄糖的转换因子。
