McNab W B, Jacobs R M, Smith H E
Animal and Plant Health Directorate, Agriculture Canada, Nepean, Ontario.
Can J Vet Res. 1994 Jan;58(1):36-41.
A chemiluminescence Western blot analysis (WBA) for detecting antibovine immunodeficiency-like virus (BIV) antibodies, had good repeatability. The test was subsequently applied to a bank of serum samples from 928 adult cows from 265 herds in Ontario; the number of cows sampled within each herd ranged from 1 to 13. The overall prevalence of anti-BIV antibodies among cows was 5.5% with a 95% confidence interval of 4.2% to 7.2%. In contrast, 18.1% of herds had at least one reactor among cows tested, resulting in a herd-prevalence confidence interval of 13.8% to 23.4%. These estimates of prevalence were in the same range as previous reports from the US and Europe. Bovine immunodeficiency-like virus may have a worldwide distribution. Unfortunately, BIV test sensitivity and specificity are difficult to estimate because virus isolation is inefficient. Therefore, the apparent prevalences could not be adjusted for test sensitivity and specificity, to estimate the true prevalence of infection. The serum samples had previously been tested for antibodies to bovine leukemia virus (BLV). There were no significant associations between BIV and BLV test results. Least squares regression was used to investigate potential associations between BIV test results and selected production indices.(ABSTRACT TRUNCATED AT 250 WORDS)
一种用于检测抗牛免疫缺陷样病毒（BIV）抗体的化学发光免疫印迹分析（WBA）具有良好的重复性。该检测随后应用于来自安大略省265个牛群的928头成年母牛的血清样本库；每个牛群中采样的母牛数量从1头到13头不等。母牛中抗BIV抗体的总体流行率为5.5%，95%置信区间为4.2%至7.2%。相比之下，18.1%的牛群在检测的母牛中有至少一头反应阳性牛，牛群流行率的置信区间为13.8%至23.4%。这些流行率估计值与美国和欧洲先前的报告范围相同。牛免疫缺陷样病毒可能在全球范围内分布。不幸的是，由于病毒分离效率低下，BIV检测的敏感性和特异性难以估计。因此，无法针对检测的敏感性和特异性调整表观流行率，以估计感染的真实流行率。这些血清样本之前已检测过抗牛白血病病毒（BLV）抗体。BIV和BLV检测结果之间没有显著关联。使用最小二乘回归来研究BIV检测结果与选定生产指标之间的潜在关联。（摘要截短于250字）
