Padegimiene E, Maneliene Z, Petruyte M, Janulaitis A
MBI Fermentas, Graiciuno 8, 2028 Vilnius, Lithuania and Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
Nucleic Acids Res. 2001 Mar 15;29(6):E30. doi: 10.1093/nar/29.6.e30.
A new type II restriction endonuclease designated OLI:I has been partially purified from the halophilic bacterium Oceanospirillum linum 4-5D. OLI:I recognizes the interrupted hexanucleotide palindrome 5'-CACNN NNGTG-3' and cleaves it in the center generating blunt-ended DNA fragments.
一种名为OLI:I的新型II型限制性内切酶已从嗜盐细菌亚麻海螺旋菌4-5D中部分纯化出来。OLI:I识别间隔的六核苷酸回文序列5'-CACNN NNGTG-3',并在中间切割,产生平端DNA片段。