Qiang B Q, Schildkraut I
Nucleic Acids Res. 1986 Mar 11;14(5):1991-9. doi: 10.1093/nar/14.5.1991.
Two new site-specific endonucleases, N1a III and N1a IV, have been isolated from Neisseria lactamica. N1a III recognizes the sequence, CATG, and cleaves 3' of the sequence to produce a four base 3' extension. N1a IV recognizes the sequence, GGNNCC, and cleaves between the two N's to produce blunt ended fragments.
已从乳酸奈瑟菌中分离出两种新的位点特异性核酸内切酶,即N1a III和N1a IV。N1a III识别序列CATG,并在该序列的3'端进行切割,产生一个四碱基的3'端延伸。N1a IV识别序列GGNNCC,并在两个N之间进行切割,产生平端片段。