Jaroszeski M J, Coppola D, Nesmith G, Pottinger C, Hyacinthe M, Benson K, Gilbert R, Heller R
Department of Surgery, MDC Box 16, College of Medicine, University of South Florida, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612-4799, USA.
Eur J Cancer. 2001 Feb;37(3):414-21. doi: 10.1016/s0959-8049(00)00407-x.
Preliminary studies that used electric pulses in vivo to facilitate entry of chemotherapeutic agents into tumour cells resulted in a 69% complete response rate for hepatocellular carcinoma in rats. This success motivated a focused investigation to define the adverse effects of this treatment on normal liver tissue. Bleomycin doses ranging from 0.5 to 2.5 U and electric fields from 500 to 2250 V/cm were investigated. Electrical treatment was administered using an array of six needles arranged in a circular pattern. Necrosis and four other histological parameters were examined 14 and 56 days after treatment. Results indicated that treatment effects were localised to the volume of treated tissue. These parameters, at both time points, were not significantly altered for liver tissue that was treated with all drug doses and electric fields of 1250 V/cm and below. Only the combination of more intense electric pulses with bleomycin produced adverse histological events in the form of localised liver necrosis at day 14. These effects were not visible at day 56. Liver function was normal through all of the treatment except for an elevation of several enzymes 1 day post-treatment.
初步研究表明，在体内使用电脉冲促进化疗药物进入肿瘤细胞，可使大鼠肝细胞癌的完全缓解率达到69%。这一成功促使人们开展了一项针对性研究，以确定该治疗方法对正常肝组织的不良影响。研究人员考察了博来霉素剂量范围为0.5至2.5单位以及电场强度为500至2250伏/厘米的情况。电疗通过以圆形排列的六根针组成的阵列进行。在治疗后14天和56天检查坏死情况以及其他四个组织学参数。结果表明，治疗效果局限于治疗组织的体积。在两个时间点，对于所有药物剂量以及电场强度为1250伏/厘米及以下的肝脏组织，这些参数均无显著变化。仅在14天时，更强的电脉冲与博来霉素联合使用会导致局部肝坏死形式的不良组织学事件。在56天时这些影响不可见。除了治疗后1天几种酶升高外，所有治疗过程中肝功能均正常。
