Roxström-Lindquist K, Faye I
Department of Genetics, University of Stockholm, S-106 91 Stockholm, Sweden.
Insect Mol Biol. 2001 Feb;10(1):77-86. doi: 10.1046/j.1365-2583.2001.00239.x.
An intracellular Drosophila protein, Yippee, was identified in a yeast interaction trap screen as physically interacting with Hyalophora cecropia Hemolin. The Yippee gene was isolated, structurally characterized, and mapped to the region 12A on the X-chromosome. Yippee contains a putative zinc-finger-like metal binding domain. It is the first characterized member of a conserved gene family of proteins present in diverse eukaryotic organisms, ranging from cellular slime mould to humans. A human cDNA clone was isolated and shown to be 76% identical to Drosophila Yippee. Yippee is ubiquitously expressed in different developmental stages of Drosophila and in different fetal tissues from human. Although the Hemolin-Yippee interaction remains to be further elucidated, the high degree of Yippee sequence conservation between a wide range of species suggests that this protein is of general importance in eukaryotes.
一种细胞内的果蝇蛋白Yippee，在酵母相互作用陷阱筛选中被鉴定为与天蚕蛾血淋巴蛋白（Hyalophora cecropia Hemolin）存在物理相互作用。Yippee基因被分离出来，进行了结构表征，并被定位到X染色体上的12A区域。Yippee含有一个假定的锌指样金属结合结构域。它是存在于从细胞黏菌到人类等多种真核生物中的一个保守蛋白基因家族中第一个被表征的成员。一个人类cDNA克隆被分离出来，并且显示与果蝇Yippee有76%的同一性。Yippee在果蝇的不同发育阶段以及人类的不同胎儿组织中均有广泛表达。尽管血淋巴蛋白与Yippee之间的相互作用仍有待进一步阐明，但Yippee在广泛物种间的高度序列保守性表明该蛋白在真核生物中具有普遍重要性。
