Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Queen Mary School, Nanchang University, Nanchang, Jiangxi 330036, P.R. China.
Oncol Rep. 2022 Jul;48(1). doi: 10.3892/or.2022.8345. Epub 2022 Jun 8.
The Yippee‑like (YPEL) gene family is composed of five members encoding a protein containing a zinc finger‑like metal‑binding domain. Due to its structure and location in cells, this domain is considered to be involved in cell multiplication and numerous types of cancer. However, the relationship between the protein and the prognosis of clear cell renal cell carcinoma (ccRCC) remains unknown. In the present study, using pan‑cancer data from the updated public database, the expression and correlation of YPEL genes in 33 types of cancer was systematically and comprehensively analyzed. The prognostic value of YPEL genes was evaluated by survival and Cox regression analysis. Considering the relationship between the tumor microenvironment and stem cell indices, the function of superoxide dismutase was evaluated. Tumor Immune Assessment Resources (TIMER) and CIBERSORT algorithm analysis were used to evaluate the correlation between YPEL genes and tumor immune infiltrating cells (TIICs). Furthermore, knockdown experiments of YPEL genes were developed to explore their effects on ccRCC cell proliferation, migration and invasion in ccRCC cell lines. Members of the YPEL family were differentially expressed in ccRCC. Increased expression levels of YPEL1, YPEL2, and YPEL5 were associated with improved overall survival and disease‑specific survival. TIMER and CIBERSORT analyses showed remarkable correlation between YPEL family members and TIICs. More importantly, the results of Cell Counting Kit‑8, EdU and Transwell assays revealed that the multiplication, migration and invasion abilities of ccRCC cell lines could be promoted by knocking out YPEL1, YPEL2 and YPEL5. In conclusion, the present study provided new insight into the different roles of YPEL1, YPEL2 and YPEL5 in ccRCC, and the relationship between YPEL1 and immune infiltration may offer new options for future clinical treatment.
YPEL 基因家族由五个成员组成，编码一种含有锌指样金属结合域的蛋白质。由于其结构和在细胞中的位置，该结构域被认为与细胞增殖和多种类型的癌症有关。然而，该蛋白与透明细胞肾细胞癌（ccRCC）的预后之间的关系尚不清楚。在本研究中，使用来自更新的公共数据库的泛癌症数据，系统和全面地分析了 33 种癌症中 YPEL 基因的表达和相关性。通过生存和 Cox 回归分析评估 YPEL 基因的预后价值。考虑到肿瘤微环境与干细胞指数之间的关系，评估了超氧化物歧化酶的功能。使用肿瘤免疫评估资源（TIMER）和 CIBERSORT 算法分析评估了 YPEL 基因与肿瘤免疫浸润细胞（TIICs）之间的相关性。此外，还开发了 YPEL 基因的敲低实验，以探讨它们对 ccRCC 细胞系中 ccRCC 细胞增殖、迁移和侵袭的影响。YPEL 家族成员在 ccRCC 中差异表达。YPEL1、YPEL2 和 YPEL5 的表达水平增加与总生存率和疾病特异性生存率的提高相关。TIMER 和 CIBERSORT 分析显示 YPEL 家族成员与 TIICs 之间存在显著相关性。更重要的是，Cell Counting Kit-8、EdU 和 Transwell 测定的结果表明，敲除 YPEL1、YPEL2 和 YPEL5 可以促进 ccRCC 细胞系的增殖、迁移和侵袭能力。总之，本研究为 YPEL1、YPEL2 和 YPEL5 在 ccRCC 中的不同作用提供了新的见解，而 YPEL1 与免疫浸润之间的关系可能为未来的临床治疗提供新的选择。
