Wu H, Li Y, Shen Z
Department of Pathology, Norman Bethune University of Medical Sciences, Changchun 130021.
Zhonghua Bing Li Xue Za Zhi. 1998 Feb;27(1):27-30.
To determine the heterogenicity in the expression of class II major histocompatible antigen (HLA-DR), factor VIII related antigen (FVIIIRAg) and the biological behavior of vessel endothelial cells in malignant lymphoma.
Standard immunohistochemical method using antiserum of HLA-DR and FVIIIRAg were applied to detect heterogeneous expression of endothelial cells in 39 cases of malignant lymphomas using neonatal lymph nodes, normal adult lymph nodes and lymph node hyperplasia as the controls.
Both HLA-DR and FVIIIRAg reactivity was found in blood vessels in neonate lymph nodes, normal adult lymph nodes and lymph node hyperplasia. Most vessels in Hodgkin's disease and T cell lymphoma had high endothelial post capillary venules (HEV), which expressed both FVIIIRAg and HLA-DR. In B cell lymphoma, most blood vessels are capillary-like microvessels, which expressed little or no HLA-DR, only expressing FVIIIRAg.
The above results indicate that the endothelial cell of blood vessels in Hodgkin's disease and T cell lymphoma not only participate in immunoregulation but also in blood coagulation, whereas, those in B cell lymphoma only participate in blood coagulation.
确定恶性淋巴瘤中Ⅱ类主要组织相容性抗原（HLA - DR）、因子Ⅷ相关抗原（FVIIIRAg）表达的异质性以及血管内皮细胞的生物学行为。
采用抗HLA - DR和FVIIIRAg抗血清的标准免疫组织化学方法，以新生儿淋巴结、正常成人淋巴结及淋巴结增生为对照，检测39例恶性淋巴瘤中内皮细胞的异质性表达。
在新生儿淋巴结、正常成人淋巴结及淋巴结增生的血管中均发现HLA - DR和FVIIIRAg反应性。霍奇金病和T细胞淋巴瘤中的大多数血管具有高内皮后微静脉（HEV），其同时表达FVIIIRAg和HLA - DR。在B细胞淋巴瘤中，大多数血管为毛细血管样微血管，几乎不表达或不表达HLA - DR，仅表达FVIIIRAg。
上述结果表明，霍奇金病和T细胞淋巴瘤中的血管内皮细胞不仅参与免疫调节，还参与血液凝固，而B细胞淋巴瘤中的血管内皮细胞仅参与血液凝固。
