Lin Y, Brosius J, Tiedge H
Department of Physiology and Pharmacology, State University of New York, Health Science Center at Brooklyn, Brooklyn, New York 11203, USA.
Neuroscience. 2001;103(2):465-79. doi: 10.1016/s0306-4522(01)00003-3.
Brain-specific cytoplasmic RNA 1 (BC1-RNA), a non-coding RNA polymerase III transcript, is a neuronal RNA that is specifically targeted to dendritic domains. It is co-localized with components of the dendritic protein synthetic machinery, and it has been suggested to operate in the regulation of local translation-related processes in postsynaptic microdomains, thus subserving long-term synaptic plasticity in neurons. To probe the relevance of BC1 expression in neuronal plasticity, we have analyzed the expression pattern of BC1 RNA in the rat nervous system. We found that BC1 RNA is expressed by a specific subset of neurons (but not by non-neuronal cells) in the central and peripheral nervous system of the adult rat. The BC1 labeling pattern indicates that the subcellular location of the RNA is typically postsynaptic which, depending on cell type, manifests itself in a predominantly somatic, somatodendritic, or dendritic location. Our results further show that BC1-expressing neurons typically co-express the messenger RNA for growth-associated protein-43 (GAP-43). Such co-expression was observed in diverse brain areas, including the olfactory bulb, neocortex, and hippocampus, among others. While BC1 RNA was in many neuronal cell types detectable in distal dendritic domains, GAP-43 messenger RNA was typically more restricted to neuronal perikarya. In the mature nervous system, expression of GAP-43 has been described as an intrinsic determinant of predominantly presynaptic plasticity, while BC1 RNA has been implicated in postsynaptic plasticity. Co-expression of both RNAs, as reported here, thus identifies a distinct subset of neurons in the rat nervous system that exhibits both types of plasticity.
脑特异性细胞质RNA 1（BC1-RNA）是一种非编码RNA聚合酶III转录本，是一种特异性定位于树突区域的神经元RNA。它与树突蛋白合成机制的成分共定位，有人提出它在调节突触后微区的局部翻译相关过程中发挥作用，从而有助于神经元的长期突触可塑性。为了探究BC1表达在神经元可塑性中的相关性，我们分析了大鼠神经系统中BC1 RNA的表达模式。我们发现，BC1 RNA由成年大鼠中枢和外周神经系统中的特定神经元亚群表达（而非非神经元细胞）。BC1标记模式表明，RNA的亚细胞定位通常是突触后，根据细胞类型的不同，表现为主要在胞体、胞体树突或树突部位。我们的结果进一步表明，表达BC1的神经元通常共表达生长相关蛋白-43（GAP-43）的信使RNA。在包括嗅球、新皮层和海马体等在内的不同脑区都观察到了这种共表达。虽然在许多神经元细胞类型的远端树突区域可检测到BC1 RNA，但GAP-43信使RNA通常更局限于神经元胞体。在成熟的神经系统中，GAP-43的表达被描述为主要突触前可塑性的内在决定因素，而BC1 RNA则与突触后可塑性有关。因此，如本文所报道的，两种RNA的共表达确定了大鼠神经系统中一个独特的神经元亚群，该亚群表现出两种类型的可塑性。
