Tiedge H, Fremeau R T, Weinstock P H, Arancio O, Brosius J
Fishberg Reseach Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2093-7. doi: 10.1073/pnas.88.6.2093.
In nerve cells, a specialized protein synthetic machinery is thought to operate in local compartments of dendrites, in particular beneath synaptic junctions, and thereby to facilitate swift adjustments of the postsynaptic protein repertoire in situ. This notion has been supported by the identification of polyribosomes and selected mRNAs in those compartments. In this study, we report the discovery of a specific RNA polymerase III transcript in dendrites. This RNA, a noncoding, 152-nucleotide-long, single-gene transcript known as BC1 RNA, is expressed almost exclusively in the nervous system. In adult rats as well as in immature rats in late developmental stages, BC1 RNA has been located in the dendrites and somata of a subset of neurons in the central and peripheral nervous system. The colocalization of BC1 RNA with dendritic mRNAs and polyribosomes may indicate a role--possibly within the functional unit of a high molecular mass ribonucleoprotein particle--in specific pre- or posttranslational processes in postsynaptic compartments of neurons.
在神经细胞中，一种特殊的蛋白质合成机制被认为在树突的局部区域发挥作用，尤其是在突触连接下方，从而促进突触后蛋白质组原位的快速调整。在这些区域发现多核糖体和特定的信使核糖核酸支持了这一观点。在本研究中，我们报告了在树突中发现一种特定的RNA聚合酶III转录本。这种RNA是一种非编码的、152个核苷酸长的单基因转录本，称为BC1 RNA，几乎只在神经系统中表达。在成年大鼠以及发育后期的未成熟大鼠中，BC1 RNA已定位在中枢和外周神经系统中一部分神经元的树突和胞体中。BC1 RNA与树突信使核糖核酸和多核糖体的共定位可能表明其在神经元突触后区域特定的翻译前或翻译后过程中发挥作用——可能是在高分子量核糖核蛋白颗粒的功能单元内。
