Department of Biochemistry and Cell Biology, University of Rzeszow, St. Pigonia 6, 35959 Rzeszów, Poland.
Cell Biol Int. 2010 Jul;34(7):731-6. doi: 10.1042/CBI20100134.
Oxidative stress during aging of Saccharomyces cerevisiae in stationary culture was documented by demonstration of progressive increase in the formation of superoxide, decrease in the content of acid-soluble thiols and of acid-soluble antioxidant capacity of cell extracts, and accumulation of aldehydes and protein carbonyl groups in two yeast strains and decreases in activities of antioxidant enzymes. Cells of a CuZn-SOD (superoxide dismutase)-1-deficient strain showed a higher loss of viability than cells of an isogenic wild-type strain. Cell survival was augmented, and changes in biochemical parameters were ameliorated, by addition of exogenous antioxidants (ascorbic acid, glutathione and melatonin) in both strains.
超氧自由基形成逐渐增加，细胞提取物中的可溶酸性硫醇和可溶酸性抗氧化能力含量降低，醛类和蛋白质羰基基团在两种酵母菌株中积累，抗氧化酶的活性降低。CuZn-SOD（超氧化物歧化酶）-1 缺陷型菌株的细胞比同种野生型菌株的细胞具有更高的失活率。通过在两种菌株中添加外源性抗氧化剂（抗坏血酸、谷胱甘肽和褪黑素），细胞存活率增加，生化参数的变化得到改善。
