评估4-硝基苯基-β-D-吡喃葡萄糖醛酸（PGUA）检测的两种快速改良方法用于从尿液中鉴定大肠杆菌。

Evaluation of two rapid modifications of the 4-nitrophenyl-beta-D-glucopyranosiduronic acid (PGUA) assay for the identification of Escherichia coli from urine.

作者信息

Dunne W M, Eisses J F

机构信息

Microbiology Division, Henry Ford Hospital, 48202, Detroit, MI, USA.

出版信息

Diagn Microbiol Infect Dis. 2001 Feb;39(2):117-9. doi: 10.1016/s0732-8893(00)00229-7.

DOI:10.1016/s0732-8893(00)00229-7

PMID:11248524

Abstract

Two rapid modifications of a tube assay for the detection of beta-glucuronidase activity (PGUA assay) were evaluated for the identification of Escherichia coli from urine cultures. A microwell and filter paper adaptation of the PGUA assay were tested using 1,234 oxidase-negative, Gram-negative rods isolated from urine on MacConkey agar in clinically significant numbers. There was perfect correlation between both methods and 676 of 797 E. coli isolates were PGUA-positive within 2 h while all of remaining isolates were PGUA-negative (sensitivity = 85%; specificity = 100%). We conclude that either modified format of the PGUA assay provides a useful, inexpensive, and rapid alternative spot test for the definitive identification of E. coli from urine because of the high degree of specificity.

摘要

为了从尿培养物中鉴定出大肠杆菌，对检测β-葡萄糖醛酸酶活性的试管检测法（PGUA检测法）的两种快速改良方法进行了评估。使用从麦康凯琼脂上尿液中分离出的1234株氧化酶阴性革兰氏阴性杆菌进行了PGUA检测法的微孔板和滤纸改良法测试，这些菌株在临床上具有显著数量。两种方法之间具有完美的相关性，797株大肠杆菌分离株中有676株在2小时内PGUA呈阳性，而其余所有分离株PGUA均为阴性（敏感性=85%；特异性=100%）。我们得出结论，由于高度特异性，PGUA检测法的任何一种改良形式都为从尿液中明确鉴定大肠杆菌提供了一种有用、廉价且快速的替代斑点试验。