一种用于从兽医标本中初步鉴定大肠杆菌的快速试管检测法的评估
Evaluation of a rapid tube assay for presumptive identification of Escherichia coli from veterinary specimens.
作者信息
Iritani B, Inzana T J
机构信息
Department of Veterinary Microbiology-Pathology, Washington State University, Pullman 99164-7040.
出版信息
J Clin Microbiol. 1988 Mar;26(3):564-6. doi: 10.1128/jcm.26.3.564-566.1988.
Abstract
Three hundred sixty-six isolates of gram-negative, oxidase-negative bacteria from veterinary specimens were tested by a tube test for identification as Escherichia coli by production within 60 min of indole, beta-galactosidase, and beta-glucuronidase. The test correctly identified 255 of 269 isolates of E. coli (95% sensitivity) and correctly indicated that 97 of 97 isolates were not E. coli (100% specificity). We conclude that production of indole, beta-galactosidase, and beta-glucuronidase as measured by a rapid tube test is useful for identification of E. coli from veterinary specimens.
摘要
对从兽医标本中分离出的366株革兰氏阴性、氧化酶阴性细菌进行了试管试验，通过检测60分钟内吲哚、β-半乳糖苷酶和β-葡萄糖醛酸酶的产生来鉴定是否为大肠杆菌。该试验正确鉴定出269株大肠杆菌中的255株（敏感性为95%），并正确表明97株非大肠杆菌分离株均不是大肠杆菌（特异性为100%）。我们得出结论，通过快速试管试验测定吲哚、β-半乳糖苷酶和β-葡萄糖醛酸酶的产生，有助于从兽医标本中鉴定大肠杆菌。
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