Jenum P A
Acta Pathol Microbiol Immunol Scand B. 1985 Dec;93(6):407-10. doi: 10.1111/j.1699-0463.1985.tb02910.x.
In our laboratory, selected strains of Gram-negative rods from urine samples are identified as Escherichia coli on the basis of smell and morphology on lactose agar. To investigate the accuracy of this routine practice, 211 consecutive strains were tested in the urea-indole tube of the Three-tube method (3-TM), in the PGUA test detecting beta-glucuronidase activity and in the Simmons' citrate test, to select the strains that were non-E. coli. Additional 1022 strains were tested by the indole and urease tests of the 3-TM only. The identification of E. coli based on the macroscopic evaluation of colonies on lactose agar gave correct results for 99.1% of the strains. Citrobacter freundii was the most frequent cause of erroneous identification.
在我们实验室，根据尿样中革兰氏阴性杆菌在乳糖琼脂上的气味和形态，将挑选出的菌株鉴定为大肠杆菌。为了研究这种常规做法的准确性，我们采用三管法（3-TM）的尿素-吲哚管、检测β-葡萄糖醛酸酶活性的PGUA试验以及西蒙氏柠檬酸盐试验，对211株连续菌株进行检测，以筛选出非大肠杆菌菌株。另外1022株菌株仅通过3-TM的吲哚和尿素酶试验进行检测。基于乳糖琼脂上菌落的宏观评估对大肠杆菌进行的鉴定，对99.1%的菌株给出了正确结果。弗氏柠檬酸杆菌是错误鉴定最常见的原因。
