Kainz A, Lubitz W, Busse H J
Institut für Mikrobiologie und Genetik, Universität Wien, Austria.
Syst Appl Microbiol. 2000 Dec;23(4):494-503. doi: 10.1016/S0723-2020(00)80023-5.
In order to investigate the relationships between species of the genus Pasteurella sensu stricto such as Pasteurella multocida, Pasteurella canis, Pasteurella stomatis, Pasteurella dagmatis, Pasteurella avium, Pasteurella volantium, Pasteurella gallinarum, Pasteurella species A, Pasteurella species B and "Pasteurella leonis" MCCM 00659 their genomic fingerprints and ARDRA profiles were compared and their quinone systems were analysed. Visual comparison of band patterns from rep-PCR (ERIC-, REP- and BOX-PCR) and the analyses of the combined band patterns by UPGMA (unweighted pair group method with averages) dendrogram derived from the combined fingerprint profiles demonstrated that each strain displays a distinct genomic fingerprint. In members of the same species several similarities in the band patterns were observed. Combined ARDRA profiles, obtained after digestion of amplified 23S rRNA coding genes with the enzymes DdeI, MseI and RsaI, revealed a dissection of the members of the genus Pasteurella sensu stricto into two groups which was in agreement with the two groups obtained from our analyses of the quinone systems. These two groups corresponded with the two phylogenetically determined subclusters 3A and 3B described previously. The species of subcluster 3A displayed a quinone system with ubiquinone Q-7 (32-56%) and ubiquinone Q-8 (44-63%) as major compounds. Members of subcluster 3B had a quinone system with ubiquinone Q-8 (86-97%) as the major compound. Based on these results it can be suggested that the genus Pasteurella sensu stricto should be restricted to the species of subcluster 3B including the species Pasteurella multocida, Pasteurella canis, Pasteurella stomatis, Pasteurella dagmatis and Pasteurella species B. In addition, evidence was found which would indicate that: 1) Pasteurella canis MCCM 00927 is misnamed and should be reclassified with Pasteurella multocida; 2) Pasteurella multocida subsp. septica may be classified as a separate species; and 3) "Pasteurella leonis" MCCM 00659 represents a separate species within subcluster 3B and thus could be described as a species of Pasteurella sensu stricto (also in a redefined genus) when more strains become available.
为了研究多杀巴斯德菌、犬巴斯德菌、口腔巴斯德菌、达氏巴斯德菌、禽巴斯德菌、飞禽巴斯德菌、鸡巴斯德菌、巴斯德菌A种、巴斯德菌B种以及“狮巴斯德菌”MCCM 00659等狭义巴斯德菌属各菌种之间的关系，对它们的基因组指纹图谱和ARDRA图谱进行了比较，并分析了它们的醌系统。通过rep-PCR（ERIC-PCR、REP-PCR和BOX-PCR）得到的条带模式的目视比较以及对由组合指纹图谱通过UPGMA（非加权平均法）构建的树状图进行分析，结果表明每个菌株都呈现出独特的基因组指纹图谱。在同一菌种的成员中，观察到条带模式有一些相似之处。用DdeI、MseI和RsaI酶消化扩增的23S rRNA编码基因后获得的组合ARDRA图谱显示，狭义巴斯德菌属的成员被分为两组，这与我们对醌系统分析得到的两组结果一致。这两组与先前描述的两个系统发育确定的亚群3A和3B相对应。亚群3A的菌种显示以泛醌Q-7（32 - 56%）和泛醌Q-8（44 - 63%）为主要成分的醌系统。亚群3B的成员具有以泛醌Q-8（86 - 97%）为主要成分的醌系统。基于这些结果，可以认为狭义巴斯德菌属应仅限于亚群3B的菌种，包括多杀巴斯德菌、犬巴斯德菌、口腔巴斯德菌、达氏巴斯德菌和巴斯德菌B种。此外，还发现了一些证据表明：1）犬巴斯德菌MCCM 00927的命名有误，应重新分类为多杀巴斯德菌；2）多杀巴斯德菌败血亚种可能应分类为一个单独的菌种；3）“狮巴斯德菌”MCCM 00659代表亚群3B内的一个单独菌种，因此当有更多菌株可用时，可以将其描述为狭义巴斯德菌属的一个菌种（也在重新定义的属中）。
