Holst E, Rollof J, Larsson L, Nielsen J P
Department of Medical Microbiology, Lund University, Sölvegatan, Sweden.
J Clin Microbiol. 1992 Nov;30(11):2984-7. doi: 10.1128/jcm.30.11.2984-2987.1992.
During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida subsp. multocida, 21 as Pasteurella multocida subsp. septica, 28 as Pasteurella canis, 10 as Pasteurella stomatis, and 5 as Pasteurella dagmatis. The homology within and between the Pasteurella species regarding cellular fatty acids and enzymatic activities was also studied. Strains of the different Pasteurella species were indistinguishable from each other regarding fatty acid composition; all strains contained major amounts of C14:0, C16:1, C16:0, and 3-OH-C14:0 acids and minor amounts of C18:2, C18:1, and C18:0 acids. Neither did the enzymatic activities distinguish between strains belonging to different species. In addition, of 56 strains examined, toxin production was demonstrated only in 1 strain each of P. multocida subsp. multocida and P. canis. Except for one severe case of necrotizing cellulitis involving P. dagmatis, P. multocida subsp. multocida or P. multocida subsp. septica was recovered in the more serious cases of infection. Except for P. canis, which in all cases was associated with dog bites, most Pasteurella strains were recovered in cases of infection associated with cat bites or scratches. Pasteurella strains occurred in four infected patients without evident connections with animals.
在3年期间，对瑞典隆德临床微生物实验室分离出的所有巴斯德氏菌菌株进行了生化研究，以确定它们与该属最近描述的分类群的关系。从146名受感染人类中分离出159株菌株，其中95株被鉴定为多杀巴斯德氏菌多杀亚种，21株为多杀巴斯德氏菌败血亚种，28株为犬巴斯德氏菌，10株为口巴斯德氏菌，5株为达氏巴斯德氏菌。还研究了不同巴斯德氏菌属内和属间细胞脂肪酸和酶活性的同源性。不同巴斯德氏菌属的菌株在脂肪酸组成上彼此无法区分；所有菌株都含有大量的C14:0、C16:1、C16:0和3-OH-C14:0酸，以及少量的C18:2、C18:1和C18:0酸。酶活性也无法区分不同种的菌株。此外，在检测的56株菌株中，仅在1株多杀巴斯德氏菌多杀亚种和1株犬巴斯德氏菌中检测到毒素产生。除了1例涉及达氏巴斯德氏菌的严重坏死性蜂窝织炎病例外，在更严重的感染病例中分离出的是多杀巴斯德氏菌多杀亚种或多杀巴斯德氏菌败血亚种。除了所有病例都与狗咬伤有关的犬巴斯德氏菌外，大多数巴斯德氏菌菌株是在与猫咬伤或抓伤相关的感染病例中分离出的。在4例感染患者中分离出巴斯德氏菌菌株，这些患者与动物没有明显关联。
