Wong J P, Zabielski M A, Schmaltz F L, Brownlee G G, Bussey L A, Marshall K, Borralho T, Nagata L P
Chemical and Biological Defence Section, Defence Research Establishment Suffield, Box 4000 Station Main, Alta, T1A 8K6, Medicine Hat, Canada.
Vaccine. 2001 Mar 21;19(17-19):2461-7. doi: 10.1016/s0264-410x(00)00474-6.
DNA vaccination using plasmid encoding the hemagglutinin (HA) gene of influenza A/PR/8/34 virus to induce long-lasting protective immunity against respiratory infection was evaluated in this study. Using liposomes as carriers, the efficacy of DNA vaccines was determined using a lethal influenza infection model in mice. Mice immunized intranasally or intramuscularly with liposome-encapsulated pCI plasmid encoding HA (pCI-HA10) were completely protected against an intranasal 5 LD(50) influenza virus challenge. Mice immunized with liposome-encapsulated pCI-HA10, but not naked pCI-HA10, by intranasal administration were found to produce high titers of serum IgA. These results suggest DNA vaccines encapsulated in liposomes are efficacious in inducing complete protective immunity against respiratory influenza virus infection.
本研究评估了使用编码甲型流感病毒A/PR/8/34血凝素（HA）基因的质粒进行DNA疫苗接种，以诱导针对呼吸道感染的持久保护性免疫。以脂质体为载体，利用小鼠致死性流感感染模型确定DNA疫苗的效力。经鼻内或肌肉内接种脂质体包裹的编码HA的pCI质粒（pCI-HA10）的小鼠完全受到保护，免受鼻内5个半数致死剂量（LD50）流感病毒的攻击。经鼻内接种脂质体包裹的pCI-HA10而非裸pCI-HA10的小鼠被发现产生高滴度的血清IgA。这些结果表明，脂质体包裹的DNA疫苗在诱导针对呼吸道流感病毒感染的完全保护性免疫方面是有效的。
