Fina F, Romain S, Ouafik L, Palmari J, Ben Ayed F, Benharkat S, Bonnier P, Spyratos F, Foekens J A, Rose C, Buisson M, Gérard H, Reymond M O, Seigneurin J M, Martin P M
Assistance Publique-Hôpitaux de Marseille, Laboratoire de Transfert d'Oncologie Biologique, Faculté de Médecine Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France.
Br J Cancer. 2001 Mar 23;84(6):783-90. doi: 10.1054/bjoc.2000.1672.
Since the few data exploring a possible association between Epstein-Barr virus (EBV) and breast cancer are conflicting, we investigated this association together with the influences of geographical areas. 509 breast cancers were sampled from areas with varying risks of nasopharynx carcinoma (NPC) such as North Africa (Algeria and Tunisia, high-risk area); southern France (Marseille, intermediate-risk area); and northern Europe (northern France, the Netherlands and Denmark; low-risk areas). Polymerase chain reaction (PCR) of a subregion of EBV BamHIC encoding the EBERs demonstrated that 31.8% of the tumours contained the viral genome. No significant differences were observed among the geographical areas. However, positive samples showed higher loads of the EBV genome in the NPC high- and intermediate-risk areas than in the low-risk areas. EBV type 1 was the dominant strain. In situ hybridization studies using a(35)S-labelled riboprobe for EBER1 and a laser capture microdissection, combined with quantitative PCR, showed that EBV localization was restricted to some tumour epithelial cell clusters. EBV could not be detected in the stroma. Considering the whole population covered, the presence of the EBV genome was not correlated with age, menopausal status, tumour, size, nodal status or histological grade.
由于探索爱泼斯坦-巴尔病毒（EBV）与乳腺癌之间可能关联的现有数据相互矛盾，我们研究了这种关联以及地理区域的影响。从鼻咽癌（NPC）风险各异的地区采集了509例乳腺癌样本，这些地区包括北非（阿尔及利亚和突尼斯，高风险地区）；法国南部（马赛，中等风险地区）；以及北欧（法国北部、荷兰和丹麦；低风险地区）。对编码EBERs的EBV BamHIC一个亚区域进行聚合酶链反应（PCR），结果显示31.8%的肿瘤含有病毒基因组。在不同地理区域之间未观察到显著差异。然而，阳性样本在NPC高风险和中等风险地区的EBV基因组载量高于低风险地区。EBV 1型是主要毒株。使用针对EBER1的α(35)S标记核糖探针进行原位杂交研究，并结合激光捕获显微切割和定量PCR，结果表明EBV定位局限于一些肿瘤上皮细胞簇。在基质中未检测到EBV。就所涵盖的总体人群而言，EBV基因组的存在与年龄绝经状态、肿瘤大小、淋巴结状态或组织学分级均无关联。
