Department of Pathology, KGMU, Lucknow, UP, India.
Department of Surgical Oncology, KGMU, Lucknow, UP, India.
Asian Pac J Cancer Prev. 2022 Nov 1;23(11):3717-3723. doi: 10.31557/APJCP.2022.23.11.3717.
Worldwide, breast cancer (BC) is a prominent cause of death, with a disproportionately high incidence in developed countries. Epstein-Barr virus (EBV) infection has been reported in up to 90% of the world's population. Although the exact link of EBV infection and breast carcinoma is not yet determined. The present study was carried out to assess the pathological correlation of EBV infection and BC in women from Northern India.
In this prospective observational study, 130 patients with histologically proven breast carcinoma were included. After detailed histology, the paraffin block with infiltrative tumor was selected for molecular analysis and further immunohistochemistry (IHC)- EBV PCR and Epstein-Barr virus latent membrane protein 1 (LMP1) IHC.
Most of the patients were diagnosed with Infiltrating Ductal Carcinoma not otherwise specified (IDC-NOS), followed by Infiltrating Ductal Carcinoma + Ductal Carcinoma in situ (IDC + DCIS). The total of 25 tissues of breast carcinoma had positive EBV PCR results (19.23%). The co-relation between the molecular and immunohistochemical results was significant in 11/25 cases that showed immunoexpression for LMP1 by IHC. Sensitivity of 44% and specificity of 100% were observed for LMP1 IHC, having a PPV value of 100% and an NPV of 88%. No significant correlation was observed between age, tumor subtype, grade, stage with respect to EBV infection; however, there was a significant association with nodal metastasis with extra nodal extension in tumors that had EBV infection.
The present study establishes an association between LMP1 and patients with EBV positive breast cancer. The authors suggest that additional multicentric studies be conducted to strengthen the reliability and generalizability of the observations of the current study.
在全球范围内，乳腺癌（BC）是导致死亡的主要原因之一，在发达国家的发病率更高。据报道，高达 90%的世界人口感染了 Epstein-Barr 病毒（EBV）。尽管 EBV 感染与乳腺癌的确切联系尚未确定。本研究旨在评估 EBV 感染与印度北部女性乳腺癌的病理相关性。
在这项前瞻性观察研究中，纳入了 130 名经组织学证实的乳腺癌患者。在详细的组织学检查后，选择有浸润性肿瘤的石蜡块进行分子分析和进一步的免疫组化（IHC）- EBV PCR 和 Epstein-Barr 病毒潜伏膜蛋白 1（LMP1）IHC。
大多数患者被诊断为非特殊型浸润性导管癌（IDC-NOS），其次是浸润性导管癌+导管原位癌（IDC+DCIS）。总共 25 份乳腺癌组织的 EBV PCR 结果呈阳性（19.23%）。在 11/25 例显示 LMP1 通过 IHC 免疫表达的病例中，分子和免疫组化结果之间存在显著相关性。LMP1 IHC 的灵敏度为 44%，特异性为 100%，PPV 值为 100%，NPV 值为 88%。EBV 感染与年龄、肿瘤亚型、分级、分期无显著相关性；然而，在有 EBV 感染的肿瘤中，与淋巴结转移和肿瘤外淋巴结扩散有显著相关性。
本研究确立了 LMP1 与 EBV 阳性乳腺癌患者之间的关联。作者建议进行更多的多中心研究，以增强当前研究观察结果的可靠性和普遍性。
