Pouzet B, Vilquin J T, Hagège A A, Scorsin M, Messas E, Fiszman M, Schwartz K, Menasché P
Department of Cardiovascular Surgery, H pital Bichat, INSERM U523, Institut de Myologie, Paris, France.
Ann Thorac Surg. 2001 Mar;71(3):844-50; discussion 850-1. doi: 10.1016/s0003-4975(00)01785-9.
This study assessed the extent to which the initial degree of functional impairment and the number of injected cells may influence the functional improvement provided by autologous skeletal myoblast transplantation into infarcted myocardium.
One week after left coronary artery ligation, 44 rats received into the infarcted scar, autologous skeletal myoblasts expanded in vitro for 7 days (mean, 3.5 x 10(6), n = 21), or culture medium alone (controls, n = 23). Left ventricular function was assessed by two-dimensional echocardiography.
When transplanted hearts were stratified according to their baseline ejection fraction, a significant improvement occurred at 2 months in the less than 25% (from 21.4% to 37%), 25% to 35% (from 29% to 43.8%), and in the 35% to 40% (from 37.2% to 41.7%) groups, compared to controls (p = 0.048, 0.0057, and 0.034, respectively), but not in the more than 40% stratum. A significant linear relationship was found between the improvement in ejection fraction and the number of injected myoblasts, both at 1 and 2 months after transplantation (p < 0.0001).
Autologous myoblast transplantation is functionally effective over a wide range of postinfarct ejection fractions, including in the sickest hearts provided that they are injected with a sufficiently high number of cells.
本研究评估了功能障碍的初始程度和注入细胞的数量对自体骨骼肌成肌细胞移植到梗死心肌后功能改善的影响程度。
在左冠状动脉结扎一周后，44只大鼠在梗死瘢痕处接受体外扩增7天的自体骨骼肌成肌细胞（平均3.5×10⁶个，n = 21），或仅接受培养基（对照组，n = 23）。通过二维超声心动图评估左心室功能。
当根据基线射血分数对移植心脏进行分层时，与对照组相比，射血分数低于25%（从21.4%升至37%）、25%至35%（从29%升至43.8%）以及35%至40%（从37.2%升至41.7%）的组在2个月时出现了显著改善（p分别为0.048、0.0057和0.034），但射血分数高于40%的层未出现显著改善。在移植后1个月和2个月时，射血分数的改善与注入的成肌细胞数量之间均发现了显著的线性关系（p < 0.0001）。
自体成肌细胞移植在广泛的梗死后射血分数范围内均具有功能有效性，包括病情最严重的心脏，前提是注入足够数量的细胞。
