Löster H, Böhm U
Institute of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Germany.
Mol Cell Biochem. 2001 Jan;217(1-2):83-90. doi: 10.1023/a:1007255021484.
The study investigated the influence of L-carnitine on the formation of malondialdehyde, an indicator of lipid peroxidation, in isolated Langendorff rat hearts. Earlier investigations of hemodynamic parameters and the recovery of ATP and creatine phosphate, carried out by means of 31P-NMR spectroscopy, had demonstrated that, depending on the composition of the perfusates (content of glucose, fatty acids, and carnitine), quite strong differences may occur in the reperfusion period after ischemia. In order to determine a possible relationship between these differences and the addition of carnitine, the study investigated whether carnitine penetrated into the tissue during the experiments, and whether it was able to reduce the concentration of detrimental substances. The concentrations of free and total carnitine as well as the malondialdehyde content as an indicator of ischemia/reperfusion damage were determined in different parts of the cardiac tissue as follows: After the Langendorff-experiments the hearts were dissected, homogenized and reconditioned; then carnitine and malondialdehyde were determined. The study included 63 hearts, which were divided into 8 different perfusion groups. Carnitine concentrations in heart tissue perfused with L-carnitine were much higher than those of the controls. Since exogenous L-carnitine and formed esters could be found in the tissue after the experiment, they must have permeated the cellular membrane rapidly. The concentrations of malondialdehyde behaved in an inverted way; as expected they were lower in carnitine-perfused hearts. The favourable effects of L-carnitine, expressed both by improved cardiac dynamics and ATP and CrP recovery in the reperfusion period, are obviously due to the fact that L-carnitine reduces ischemic damage.
该研究调查了左旋肉碱对离体Langendorff大鼠心脏中丙二醛（脂质过氧化指标）形成的影响。早期通过31P-NMR光谱法对血流动力学参数以及ATP和磷酸肌酸恢复情况的研究表明，根据灌注液的组成（葡萄糖、脂肪酸和肉碱的含量），缺血后再灌注期可能会出现相当大的差异。为了确定这些差异与肉碱添加之间的可能关系，该研究调查了实验过程中肉碱是否渗透到组织中，以及它是否能够降低有害物质的浓度。在心脏组织的不同部位测定了游离和总肉碱的浓度以及作为缺血/再灌注损伤指标的丙二醛含量，具体如下：在Langendorff实验后，将心脏解剖、匀浆并进行处理；然后测定肉碱和丙二醛。该研究包括63颗心脏，分为8个不同的灌注组。用左旋肉碱灌注的心脏组织中的肉碱浓度远高于对照组。由于实验后在组织中发现了外源性左旋肉碱和形成的酯，它们一定是迅速渗透过细胞膜的。丙二醛的浓度表现出相反的趋势；正如预期的那样，在肉碱灌注的心脏中较低。左旋肉碱的有益作用，表现为再灌注期心脏动力学改善以及ATP和CrP恢复，显然是由于左旋肉碱减少了缺血损伤。
