Yoshida A, Nakano Y, Yamashita Y, Oho T, Ito H, Kondo M, Ohishi M, Koga T
Department of Oral and Maxillofacial Oncology, Kyushu University, Faculty of Dental Science, Fukuoka, Japan.
J Dent Res. 2001 Jan;80(1):346-50. doi: 10.1177/00220345010800010901.
Bacterial heat shock proteins have been implicated in the pathogenesis of several diseases, and the immunological relationship between rheumatoid arthritis (RA) and Escherichia coli DnaJ has been reported. Since there are similarities in the tissue destruction process of RA and periodontitis, we examined the reactivities of antibodies in sera from RA patients to the DnaJ protein from Actinobacillus actinomycetemcomitans. An enzyme-linked immunosorbent assay showed that IgG titers to the N-terminal conservative region of the DnaJ are significantly higher in RA patients compared with the healthy controls (p < 0.05). Furthermore, we examined IgG titers of disease controls to determine the specificity of the immune responses to this region in RA patients. The difference between RA and infectious disease patients was also significant (p < 0.05). These results suggest that the N-terminal region of DnaJ from A. actinomycetemcomitans may contribute to the etiologic analysis of RA.
细菌热休克蛋白与多种疾病的发病机制有关，且已有关于类风湿关节炎（RA）与大肠杆菌DnaJ之间免疫关系的报道。由于RA和牙周炎在组织破坏过程中有相似之处，我们检测了RA患者血清中抗体对伴放线放线杆菌DnaJ蛋白的反应性。酶联免疫吸附测定显示，与健康对照相比，RA患者针对DnaJ N端保守区域的IgG滴度显著更高（p<0.05）。此外，我们检测了疾病对照的IgG滴度，以确定RA患者对该区域免疫反应的特异性。RA患者与传染病患者之间的差异也具有显著性（p<0.05）。这些结果表明，伴放线放线杆菌DnaJ的N端区域可能有助于RA的病因分析。
