通过半巢式聚合酶链反应检测猫免疫缺陷病毒前病毒
Detection of feline immunodeficiency provirus by seminested polymerase chain reaction.
作者信息
Celer V, Kulhánková H, Celer V
机构信息
Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University, Brno, Czechia.
出版信息
Folia Microbiol (Praha). 2000;45(2):161-5. doi: 10.1007/BF02817417.
Specific primers for the detection of the FIV provirus in peripheral blood mononuclear cells (PBMC) by seminested PCR (snPCR) were developed. Forty cats (patients from veterinary hospitals) were investigated for the presence of FIV serum antibodies by immunoblot and for the presence of provirus in PBMC by snPCR. Seventeen of the 40 examined samples (42.5%) showed the presence of antibodies against FIV. The total number of animals that were found positive in snPCR was 19 (47.5%). Fourteen of the seropositive animals (35%) were positive by snPCR whereas three seropositive animals (7.5%) turned out to be snPCR negative. Of twenty-three animals that were negative by immunoblot, five (12.5%) were found to be positive by snPCR.
开发了用于通过半巢式PCR（snPCR）检测外周血单核细胞（PBMC）中猫免疫缺陷病毒（FIV）前病毒的特异性引物。通过免疫印迹法对40只猫（来自兽医医院的病猫）进行FIV血清抗体检测，并通过snPCR检测PBMC中前病毒的存在情况。40份检测样本中有17份（42.5%）显示存在抗FIV抗体。snPCR检测呈阳性的动物总数为19只（47.5%）。14只血清阳性动物（35%）snPCR检测呈阳性，而3只血清阳性动物（7.5%）snPCR检测呈阴性。在23只免疫印迹检测呈阴性的动物中，5只（12.5%）snPCR检测呈阳性。
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