Kattar M M, Cookson B T, Carlson L D, Stiglich S K, Schwartz M A, Nguyen T T, Daza R, Wallis C K, Yarfitz S L, Coyle M B
Department of Laboratory Medicine, Division of Infectious Disease, University of Washington, Seattle, Washington, USA.
J Clin Microbiol. 2001 Apr;39(4):1467-76. doi: 10.1128/JCM.39.4.1467-1476.2001.
We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. This isolate was compared with 14 other strains including reference strains of Tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, DNA-DNA hybridization, and sequencing of the 16S rRNA gene. This isolate represents a new taxon within the genus Tsukamurella for which we propose the name Tsukamurella strandjordae sp. nov. Our study also revealed that Tsukamurella paurometabola ATCC 25938 represents a misnamed Tsukamurella inchonensis isolate and confirms that Tsukamurella wratislaviensis belongs to the genus Rhodococcus.
我们从一名患有急性髓性白血病的5岁女孩的血液培养物中分离出了一种革兰氏阳性、弱抗酸酒精、不规则杆状细菌。通过基于生理和生化特性、全细胞短链脂肪酸和分枝菌酸分析、DNA-DNA杂交以及16S rRNA基因测序的多相方法,将该分离株与包括冢村氏菌属参考菌株在内的其他14个菌株进行了比较。该分离株代表了冢村氏菌属内的一个新分类单元,我们将其命名为新种冢村氏斯特兰德乔德菌(Tsukamurella strandjordae sp. nov.)。我们的研究还表明,迟缓冢村氏菌(Tsukamurella paurometabola)ATCC 25938代表了一株误命名的仁川冢村氏菌(Tsukamurella inchonensis)分离株,并证实了华沙冢村氏菌(Tsukamurella wratislaviensis)属于红球菌属。