Butler W Ray, Floyd Margaret M, Brown June M, Toney Sean R, Daneshvar Maryam I, Cooksey Robert C, Carr Janice, Steigerwalt Arnold G, Charles Nadege
Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1615-1624. doi: 10.1099/ijs.0.63465-0.
Four strains of novel, rapidly growing, acid-alcohol-fast-staining bacteria were characterized with a polyphasic approach. Isolates were received by the Centers for Disease Control and Prevention from domestic health department laboratories for reference testing as unidentifiable, clinical mycobacteria. Bacteria were rod-shaped and produced non-pigmented (white to beige), non-photochromogenic, smooth or wrinkled-rough colonies on Middlebrook 7H10 and 7H11 media at 33 degrees C. The smooth and wrinkled colony forms were representative of two species with 68.0 and 72.0 mol% DNA G+C content. The cell wall contained meso-diaminopimelic acid and mycolic acids. Species were characterized by cellular fatty acids of C10:0, C14:0, C16:1omega9t, C16:0, C18:1omega9c and 10-methyl C18:0 (tuberculostearic acid). HPLC analysis of mycolic acids produced a novel late-emerging, genus-specific mycolate pattern. TLC analysis demonstrated a novel alpha(+)-mycolate. Species were 98.9% similar by comparison of 16S rRNA gene sequences; however, the DNA-DNA association was <28 %. Phylogenetic analysis of 16S rRNA gene sequences demonstrated an association with Rhodococcus equi, although a DNA-DNA relatedness value of 2% did not support a close relationship. PCR analysis of a proposed, selected actinomycete-specific 439 bp fragment of the 65 kDa heat-shock protein was negative for three of the four isolates. The creation of Segniliparaceae fam. nov. is proposed to encompass the genus Segniliparus gen. nov., including two novel species, the type species Segniliparus rotundus sp. nov. and Segniliparus rugosus sp. nov., with the respective type strains CDC 1076(T) (=ATCC BAA-972(T)=CIP 108378(T)) and CDC 945(T) (=ATCC BAA-974(T)=CIP 108380(T)).
采用多相分类方法对4株新型、快速生长、抗酸酒精染色的细菌进行了鉴定。美国疾病控制与预防中心从国内卫生部门实验室收到这些分离株，作为无法鉴定的临床分枝杆菌进行参考检测。细菌呈杆状，在33℃的Middlebrook 7H10和7H11培养基上产生无色素（白色至米色）、非光产色、光滑或皱-粗糙型菌落。光滑型和皱型菌落形态代表两个物种，其DNA G+C含量分别为68.0 mol%和72.0 mol%。细胞壁含有内消旋二氨基庚二酸和分枝菌酸。这些物种的特征性细胞脂肪酸为C10:0、C14:0、C16:1ω9t、C16:0、C18:1ω9c和10-甲基C18:0（结核硬脂酸）。分枝菌酸的HPLC分析产生了一种新的晚期出现的属特异性分枝菌酸模式。TLC分析显示了一种新的α(+)-分枝菌酸。通过16S rRNA基因序列比较，这些物种的相似性为98.9%；然而，DNA-DNA杂交率<28%。16S rRNA基因序列的系统发育分析表明与马红球菌有关联，尽管2%的DNA-DNA相关性值不支持密切关系。对提议的、选定的放线菌特异性65 kDa热休克蛋白的439 bp片段进行PCR分析，4株分离株中有3株呈阴性。建议创建新科Segniliparaceae，包含新属Segniliparus，包括两个新物种，模式种圆拟无枝菌酸菌Segniliparus rotundus sp. nov.和皱拟无枝菌酸菌Segniliparus rugosus sp. nov.，其模式菌株分别为CDC 1076(T)（=ATCC BAA-972(T)=CIP 108378(T)）和CDC 945(T)（=ATCC BAA-974(T)=CIP 108380(T)）。
