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与灰葡萄孢感染的菜豆(菜豆属)软腐病相关的氧化应激标志物

Markers for oxidative stress associated with soft rots in French beans (Phaseolus vulgaris) infected by Botrytis cinerea.

作者信息

Muckenschnabel I, Williamson B, Goodman B A, Lyon G D, Stewart D, Deighton N

机构信息

Scottish Crop Research Institute, Invergowrie, Dundee, UK.

出版信息

Planta. 2001 Feb;212(3):376-81. doi: 10.1007/s004250000401.

DOI:10.1007/s004250000401
PMID:11289602
Abstract

The role of active oxygen species has been studied in spreading soft-rot lesions caused by the necrotrophic fungal pathogen Botrytis cinerea Pers.:Fr. in leaves of four genotypes of French bean (Phaseolus vulgaris L.). Large increases were observed for the aldehydic end-products of oxidative damage, malondialdehyde and 4-hydroxy-2-nonenal, as a result of infection in each of the genotypes studied. Similar increases were found in a stable free radical and g=4.27 Fe(III) signals, but not Mn(II) signals, in electron paramagnetic resonance spectra. These changes were accompanied by large decreases in ascorbic acid levels, with changes in the antioxidant glutathione being genotype dependent.

摘要

已对活性氧物质在由坏死性真菌病原体灰葡萄孢菌(Botrytis cinerea Pers.:Fr.)引起的菜豆(Phaseolus vulgaris L.)四个基因型叶片软腐病斑扩展中的作用进行了研究。在所研究的每个基因型中,由于感染,氧化损伤的醛类终产物丙二醛和4-羟基-2-壬烯醛均大幅增加。在电子顺磁共振光谱中,稳定自由基和g = 4.27的铁(III)信号也出现类似增加,但锰(II)信号未增加。这些变化伴随着抗坏血酸水平的大幅下降,抗氧化剂谷胱甘肽的变化则因基因型而异。

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