Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
Methods Mol Biol. 2024;2798:79-100. doi: 10.1007/978-1-0716-3826-2_6.
Malondialdehyde is a three-carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation widely used as a marker of the extent of lipid peroxidation in plants. There are several methodological approaches to quantify malondialdehyde contents in higher plants, ranging from the simplest, cheapest, and quickest spectrophotometric approaches to the more complex ones using tandem mass spectrometry. This chapter summarizes the advantages and limitations of approaches followed and provides brief protocols with some tips to facilitate the selection of the best method for each experimental condition and application.
丙二醛是一种三碳二醛，作为多不饱和脂肪酸过氧化的副产物广泛产生，通常被用作植物中脂质过氧化程度的标志物。有几种方法可用于定量测定高等植物中的丙二醛含量，从最简单、最便宜、最快的分光光度法到使用串联质谱法的更复杂方法都有。本章总结了所采用方法的优缺点，并提供了简要的方案和一些提示，以方便根据每种实验条件和应用选择最佳方法。
