Duarte M E, Noseda D G, Noseda M D, Tulio S, Pujol C A, Damonte E B
Departamento de Bioquímica, Universidade Federal do Paraná, Centro Politécnico, Curitiba, Brasil.
Phytomedicine. 2001 Jan;8(1):53-8. doi: 10.1078/0944-7113-00007.
Sulfated polysaccharides exhibit many biological properties such as antiviral and anticoagulant activities. Herein, we report the antiviral activity of sulfated galactans extracted from the red sea-weed Bostrychia montagnei against herpes simplex virus types 1 (strain F and the thymidine kinase-deficient strains Field and B2006) and 2 (strain G). Two crude extracts obtained with cold and hot water as well as some fractions obtained by anion exchange chromatography, inhibited significantly the replication of the different strains of herpesviruses as determined by plaque reduction assays. The inhibitory effect of the compounds studied here took place only when they were added during the adsorption period. They were found to be highly selective antiviral substances, causing no impairment of Vero cell viability. Furthermore, they had no direct inactivating effect on virions by incubation in a virucidal assay. The antiviral activity could be correlated with the molecular weight and sulfate content of the polysaccharides. Although sulfated polysaccharides are generally endowed with anticoagulant properties, the results of the activated partial thromboplastin time and the thrombine time assays indicated that the natural sulfated polysaccharides from Bostrychia montagnei have very low anticoagulant activity, confirming that there is no relation between the antiviral and anticoagulant properties.
硫酸化多糖具有许多生物学特性，如抗病毒和抗凝血活性。在此，我们报告从红藻波氏藻中提取的硫酸化半乳聚糖对1型单纯疱疹病毒（F株以及胸苷激酶缺陷型的Field株和B2006株）和2型单纯疱疹病毒（G株）的抗病毒活性。用冷水和热水获得的两种粗提物以及通过阴离子交换色谱法得到的一些组分，通过蚀斑减少试验测定，显著抑制了不同株单纯疱疹病毒的复制。此处研究的化合物的抑制作用仅在吸附期添加时才会发生。它们被发现是高度选择性的抗病毒物质，不会损害Vero细胞的活力。此外，在杀病毒试验中孵育时，它们对病毒粒子没有直接灭活作用。抗病毒活性可能与多糖的分子量和硫酸根含量相关。尽管硫酸化多糖通常具有抗凝血特性，但活化部分凝血活酶时间和凝血酶时间试验结果表明，来自波氏藻的天然硫酸化多糖具有非常低的抗凝血活性，证实了抗病毒和抗凝血特性之间没有关联。
