Pujol C A, Estevez J M, Carlucci M J, Ciancia M, Cerezo A S, Damonte E B
Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
Antivir Chem Chemother. 2002 Mar;13(2):83-9. doi: 10.1177/095632020201300202.
A novel series of DL-galactan hybrids extracted from the red seaweed Gymnogongrus torulosus, was evaluated for its in vitro antiviral properties against herpes simplex virus type 2 (HSV-2) and dengue virus 2 (DEN-2). These compounds were very active against both viruses with inhibitory concentration 50% (IC50) values in the range 0.6-16 microg/ml for HSV-2 and 0.19-1.7 microg/ml for DEN-2, respectively, as determined in a virus plaque reduction assay in Vero cells. The DL-galactans lacked of cytotoxic effects, on stationary as well as on actively dividing cells, and anticoagulant properties. Some of the compounds showed a variable level of direct inactivating effect on both virions, with virucidal concentration 50% values exceeding the IC50s obtained by plaque reduction assay. Full inhibitory activity was achieved when the galactans were present during virus adsorption period, suggesting that the mode of action of these compounds is an interference in the binding of the surface envelope glycoprotein with the cell receptor.
从红藻Gymnogongrus torulosus中提取的一系列新型DL-半乳聚糖杂化物，对其抗2型单纯疱疹病毒（HSV-2）和登革病毒2型（DEN-2）的体外抗病毒特性进行了评估。在Vero细胞的病毒空斑减少试验中测定，这些化合物对两种病毒都非常有效，HSV-2的50%抑制浓度（IC50）值在0.6-16微克/毫升范围内，DEN-2的IC50值在0.19-1.7微克/毫升范围内。DL-半乳聚糖对静止细胞和活跃分裂细胞均无细胞毒性作用，也没有抗凝血特性。一些化合物对两种病毒粒子均表现出不同程度的直接灭活作用，其50%杀病毒浓度值超过了通过空斑减少试验获得的IC50值。当半乳聚糖在病毒吸附期存在时可实现完全抑制活性，这表明这些化合物的作用方式是干扰表面包膜糖蛋白与细胞受体的结合。
