Oka T, Nakagomi T, Nakagomi O
Department of Microbiology, Akita University School of Medicine, Akita, Japan.
Microbiol Immunol. 2001;45(2):173-7. doi: 10.1111/j.1348-0421.2001.tb01277.x.
Nonstructural glycoprotein NSP4 of group A rotavirus has recently been shown to be a viral enterotoxin, inducing diarrhea in neonatal mice. Literature is conflicting as to whether there is any consistent amino acid substitution between virulent (or symptomatic) and attenuated (or asymptomatic) rotavirus strains. We have sequenced and compared the NSP4 sequences derived from a total of 10 geographically- and serologically-related feline rotavirus strains from both diarrheal and asymptomatically-infected kittens. These NSP4 sequences were closely related to each other and there were differences at 19 amino acid residues, but none was segregated according to whether the strain was isolated from a diarrheal kitten or not. Thus, this study failed to lend support to the contention that mutations in NSP4 play a significant role in the pathogenesis of rotavirus diarrhea. Involvement of other genes may explain the outcome of infection in cats from which these 10 feline rotaviruses were isolated.
最近研究表明，A组轮状病毒的非结构糖蛋白NSP4是一种病毒肠毒素，可在新生小鼠中诱发腹泻。关于毒性（或有症状）和减毒（或无症状）轮状病毒株之间是否存在一致的氨基酸替代，文献报道存在矛盾。我们对来自腹泻和无症状感染小猫的总共10株地理和血清学相关的猫轮状病毒株的NSP4序列进行了测序和比较。这些NSP4序列彼此密切相关，在19个氨基酸残基处存在差异，但没有一个是根据该毒株是否从腹泻小猫中分离出来而区分的。因此，本研究未能支持NSP4突变在轮状病毒腹泻发病机制中起重要作用的论点。其他基因的参与可能解释了分离出这10株猫轮状病毒的猫的感染结果。
