Iturriza-Gòmara Miren, Anderton Emma, Kang Gagandeep, Gallimore Chris, Phillips Wendy, Desselberger Ulrich, Gray Jim
Enteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory, Health Protection Agency, London, United Kingdom.
J Clin Microbiol. 2003 Aug;41(8):3566-73. doi: 10.1128/JCM.41.8.3566-3573.2003.
NSP4-encoding genes of 78 human rotavirus strains of common or reassortant genotypes were characterized by reverse transcription-PCR followed by sequencing and phylogenetic analysis. It was found that all the human strains characterized clustered into only two of the five known NSP4 genotypes. Linkage between NSP4 genotypes and VP6 subgroups was 100%, NSP4 genotype A being linked to VP6 of subgroup I (SGI) and NSP4 of genotype B being linked to VP6 of SGII. The diversity among the NSP4- and VP6-encoding genes was significantly less than that among the VP7 and VP4 genes in cocirculating human rotavirus strains. Whereas G and P types appear to be shared among different animal species and humans, the NSP4- and VP6-encoding genes appear to segregate according to their host of origin, suggesting that these two proteins may be host restriction determinants. The NSP4-VP6 association may be structurally determined during rotavirus replication (morphogenesis).
通过逆转录聚合酶链反应（RT-PCR），随后进行测序和系统发育分析，对78株常见或重组基因型的人轮状病毒株的NSP4编码基因进行了特征分析。结果发现，所有已鉴定的人毒株仅聚集在五个已知NSP4基因型中的两个。NSP4基因型与VP6亚组之间的关联度为100%，NSP4基因型A与I亚组（SGI）的VP6相关联，基因型B的NSP4与SGII的VP6相关联。在共同流行的人轮状病毒株中，NSP4和VP6编码基因之间的多样性明显低于VP7和VP4基因之间的多样性。虽然G型和P型似乎在不同动物物种和人类之间共享，但NSP4和VP6编码基因似乎根据其起源宿主而分离，这表明这两种蛋白质可能是宿主限制决定因素。NSP4-VP6关联可能在轮状病毒复制（形态发生）过程中由结构决定。
