酿酒酵母Elongator突变赋予对乳酸克鲁维酵母酶菌素的抗性。
Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin.
作者信息
Frohloff F, Fichtner L, Jablonowski D, Breunig K D, Schaffrath R
机构信息
Institut für Genetik, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 10, D-06120 Halle (Saale), Germany.
出版信息
EMBO J. 2001 Apr 17;20(8):1993-2003. doi: 10.1093/emboj/20.8.1993.
Kluyveromyces lactis killer strains secrete a zymocin complex that inhibits proliferation of sensitive yeast genera including Saccharomyces cerevisiae. In search of the putative toxin target (TOT), we used mTn3:: tagging to isolate zymocin-resistant tot mutants from budding yeast. Of these we identified the TOT1, TOT2 and TOT3 genes (isoallelic with ELP1, ELP2 and ELP3, respectively) coding for the histone acetyltransferase (HAT)-associated Elongator complex of RNA polymerase II holoenzyme. Other than the typical elp ts-phenotype, tot phenocopies hypersensitivity towards caffeine and Calcofluor White as well as slow growth and a G(1) cell cycle delay. In addition, TOT4 and TOT5 (isoallelic with KTI12 and IKI1, respectively) code for components that associate with ELONGATOR: Intriguingly, strains lacking non-Elongator HATs (gcn5, hat1, hpa3 and sas3) or non-Elongator transcription elongation factors TFIIS (dst1) and Spt4p (spt4) cannot confer resistance towards the K.lactis zymocin, thus providing evidence that Elongator equals TOT and that Elongator plays an important role in signalling toxicity of the K.lactis zymocin.
乳酸克鲁维酵母杀伤菌株分泌一种酶复合物，可抑制包括酿酒酵母在内的敏感酵母属的增殖。为了寻找假定的毒素靶点（TOT），我们使用mTn3标签从芽殖酵母中分离出对酶复合物具有抗性的tot突变体。在这些突变体中，我们鉴定出了TOT1、TOT2和TOT3基因（分别与ELP1、ELP2和ELP3等位），它们编码与RNA聚合酶II全酶的组蛋白乙酰转移酶（HAT）相关的延伸因子复合物。除了典型的elp温度敏感型表型外，tot突变体还表现出对咖啡因和荧光增白剂的超敏反应、生长缓慢以及G1期细胞周期延迟。此外，TOT4和TOT5（分别与KTI12和IKI1等位）编码与延伸因子复合物相关的成分：有趣的是，缺乏非延伸因子HAT（gcn5、hat1、hpa3和sas3）或非延伸因子转录延伸因子TFIIS（dst1）和Spt4p（spt4）的菌株对乳酸克鲁维酵母酶复合物不具有抗性，因此证明延伸因子复合物等同于TOT，并且延伸因子复合物在乳酸克鲁维酵母酶复合物的毒性信号传导中起重要作用。
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