Villani F, Aponte M, Blaiotta G, Mauriello G, Pepe O, Moschetti G
Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare ed Ambientale e di Igiene, Università degli Studi di Napoli 'Federico II', Portici, Italy.
J Appl Microbiol. 2001 Mar;90(3):430-9. doi: 10.1046/j.1365-2672.2001.01261.x.
The identification of a bacteriocin-producing lactococcal strain isolated from raw cow's milk is reported, along with production conditions, physical and chemical properties, and mode of action of the bacteriocin.
On the basis of resistance to clindamycin, species-specific PCR and amplification of the 16S-23S rDNA spacer region, the strain was identified as Lactococcus garvieae. Its bacteriocin, designated garviecin L1-5, was bactericidal against closely related species and strains of species from different genera, including Listeria monocytogenes and Clostridium spp. Garviecin L1-5 was shown to be proteinaceous by protease inactivation and was unaffected by heat treatments, also at low pH values. When amplifying known lactococcal bacteriocin genes using DNA from strain L1-5 as template, no amplification products were observed on the agarose gel. The molecular weight of garviecin L1-5 was about 2.5 kDa. As far as is known, no bacteriocins have been detected from Lactococcus garvieae.
The general properties of garviecin L1-5 are characteristic of the low-molecular-weight bactericidal peptide group.
The survey of micro-organisms for novel antimicrobial substances provided valuable information on their physiology, ecology and practical application.
报道从生牛奶中分离出的一株产细菌素的乳球菌菌株，以及该细菌素的生产条件、理化性质和作用方式。
基于对克林霉素的抗性、种特异性PCR以及16S - 23S rDNA间隔区的扩增，该菌株被鉴定为格氏乳球菌。其产生的细菌素命名为格氏菌素L1 - 5，对密切相关的物种以及来自不同属的物种菌株具有杀菌作用，包括单核细胞增生李斯特菌和梭菌属。蛋白酶失活表明格氏菌素L1 - 5是蛋白质类物质，并且在低pH值下，热处理对其也没有影响。以菌株L1 - 5的DNA为模板扩增已知的乳球菌细菌素基因时，琼脂糖凝胶上未观察到扩增产物。格氏菌素L1 - 5的分子量约为2.5 kDa。据了解，尚未从格氏乳球菌中检测到细菌素。
格氏菌素L1 - 5的一般特性具有低分子量杀菌肽组的特征。
对微生物新型抗菌物质的研究为其生理学、生态学和实际应用提供了有价值的信息。
