Patel Prittesh, Patel Bhavika, Amaresan Natarajan, Joshi Bhrugesh, Shah Rushabh, Krishnamurthy Ramar
C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat 394 350 Gujarat, India.
Biotechnol Rep (Amst). 2020 Nov 10;28:e00555. doi: 10.1016/j.btre.2020.e00555. eCollection 2020 Dec.
This study focused on agro-industrial waste such as fruit peels by extracting prebiotics as a carbon source for lactic acid bacteria (LAB). Four strains of LAB were selected from (B2 and B3) and (R4 and R5), and identified as through 16S rRNA gene sequencing. The analysis of probiotic characteristics revealed that all four strains were able to tolerate sodium chloride (up to 7 %), bile salt (up to 3 %), and broad range of pH (2-9). Further, analysis of polysaccharide contents in the agro-industrial waste materials such as peels of pineapple, orange, lemon, sugarcane, pomegranate, and sweet lemon revealed that the concentration ranged from 3.91-163.85 mg/g. It was observed that orange peels (20.38-140.99 mg/g), sweet lemon peels (22.03-161.93 mg/g), and pomegranate peels (38.19-163.85 mg/g) yielded maximum indigestible polysaccharide. Evaluation of synbiotic combination of probiotic and prebiotic revealed that strains had better fermentation efficiency with orange, sweet lemon, and pineapple compared to lemon, sugarcane, and pomegranate. In nutshell, different types of agro-industrial waste evaluated in this research were found to be a cheap and fermentable carbon sources for LAB. Further study should be conducted to analyze this symbiotic combination as feed supplements for fish in aquaculture as well as various fermentation industries.
本研究聚焦于农业工业废弃物，如果皮，通过提取益生元作为乳酸菌（LAB）的碳源。从[具体来源1]（B2和B3）和[具体来源2]（R4和R5）中选择了四株乳酸菌，并通过16S rRNA基因测序鉴定为[具体菌种]。益生菌特性分析表明，所有四株菌株都能够耐受氯化钠（高达7%）、胆盐（高达3%）以及广泛的pH范围（2 - 9）。此外，对菠萝、橙子、柠檬、甘蔗、石榴和甜橙等农业工业废弃物中的多糖含量分析表明，其浓度范围为3.91 - 163.85 mg/g。观察到橙皮（20.38 - 140.99 mg/g）、甜橙皮（22.03 - 161.93 mg/g）和石榴皮（38.19 - 163.85 mg/g）产生的难消化多糖最多。益生菌与益生元的合生元组合评估表明，与柠檬、甘蔗和石榴相比，[具体菌株]在橙子、甜橙和菠萝上具有更好的发酵效率。简而言之，本研究中评估的不同类型农业工业废弃物被发现是乳酸菌廉价且可发酵的碳源。应进一步开展研究，分析这种合生元组合作为水产养殖中鱼类饲料补充剂以及各种发酵工业的应用。
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