Angelopoulou D, Demetzos C, Dimas C, Perdetzoglou D, Loukis A
Planta Med. 2001 Mar;67(2):168-71. doi: 10.1055/s-2001-11497.
Essential oils and hexane extracts from Cistus monspeliensis L. leaves and fruits were analysed by GC-MS. Manoyl oxide and its isomers, 3 beta-hydroxy-manoyl oxide, 3 beta-hydroxy-13-epi-manoyl oxide, as well as 3 beta-acetoxy-13-epi-manoyl oxide were detected for the first time in C. monspeliensis L. Ent-13-epimanoyl oxide was isolated from the hexane extract of leaves and its structure was determined using spectroscopic methods. In vitro cytotoxic activity of ent-13-epi-manoyl oxide and mixtures of manoyl oxide isomers at 10(-4) M concentrations ranged from 11.1 to 32.2% of the activity for a vinblastine control evaluated against nine leukemic cell lines. The results showed that the manoyl oxide isomer mixtures as well as ent-13-epi-manoyl oxide exhibited a slight growth inhibiting activity.
采用气相色谱-质谱联用（GC-MS）技术对来自蒙氏岩蔷薇（Cistus monspeliensis L.）叶片和果实的精油及己烷提取物进行了分析。首次在蒙氏岩蔷薇中检测到了氧化异曼诺醇及其异构体、3β-羟基氧化异曼诺醇、3β-羟基-13-表氧化异曼诺醇以及3β-乙酰氧基-13-表氧化异曼诺醇。从叶片的己烷提取物中分离得到了对映-13-表氧化异曼诺醇，并通过光谱方法确定了其结构。在10⁻⁴ M浓度下，对映-13-表氧化异曼诺醇和氧化异曼诺醇异构体混合物对9种白血病细胞系的体外细胞毒性活性范围为长春碱对照活性的11.1%至32.2%。结果表明，氧化异曼诺醇异构体混合物以及对映-13-表氧化异曼诺醇表现出轻微的生长抑制活性。
