Anastasaki T, Demetzos C, Perdetzoglou D, Gazouli M, Loukis A, Harvala C
School of Pharmacy, Department of Pharmacognosy, University of Athens, Greece.
Planta Med. 1999 Dec;65(8):735-9. doi: 10.1055/s-1999-14095.
The qualitative and quantitative analysis of labdane-type diterpenes of the hexane extracts and of the essential oils of the leaves, fruits and resin "Ladano", of Cistus creticus subsp. creticus and Cistus creticus subsp. eriocephalus, have been carried out by GC and GC-MS analysis using two capillary chromatographic columns, i.e., HP-5MS and CP-Wax. The methanolic extract of the fruits of C. creticus subsp. creticus was examined and seven labdane diterpenes were isolated and identified by spectroscopic methods. Data on the investigation of labdane diterpenes by GC and GC-MS is limited and most of them have never been analysed by this method. The results obtained by this analysis could be useful for identifying them in crude plant extracts. Manoyl oxides were studied further for the percentage content of their isomers. The hexane extracts of the two subspecies as well as the manoyl oxide isomers isolated from the methanolic extract of the fruits of C. creticus subsp. creticus, were tested for their antimicrobial activity against Gram-positive and Gram-negative bacteria. Global numerical differences of these C. creticus subspecies, based on labdane diterpenes content in the hexane extracts as well as in the essential oils, were established by statistical methods. Phenotypic differences are discussed.
采用气相色谱（GC）和气相色谱-质谱联用（GC-MS）分析法，使用两根毛细管色谱柱，即HP-5MS和CP-Wax，对克里特岩蔷薇（Cistus creticus subsp. creticus）和毛头岩蔷薇（Cistus creticus subsp. eriocephalus）的叶子、果实和树脂“拉达诺”（Ladano）的己烷提取物及挥发油中的半日花烷型二萜进行了定性和定量分析。对克里特岩蔷薇亚种果实的甲醇提取物进行了检测，通过光谱法分离并鉴定了7种半日花烷二萜。通过GC和GC-MS对半日花烷二萜进行研究的数据有限，其中大多数从未用该方法进行分析。该分析所得结果可能有助于在粗植物提取物中鉴定它们。进一步研究了异曼oyl氧化物异构体的百分含量。测试了这两个亚种的己烷提取物以及从克里特岩蔷薇亚种果实甲醇提取物中分离出的异曼oyl氧化物异构体对革兰氏阳性菌和革兰氏阴性菌的抗菌活性。通过统计方法确定了基于己烷提取物和挥发油中半日花烷二萜含量的这些克里特岩蔷薇亚种的整体数值差异。讨论了表型差异。
