de Miguel T, Sieiro C, Poza M, Villa T G
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain.
J Agric Food Chem. 2001 Mar;49(3):1200-2. doi: 10.1021/jf001169z.
A collection of 43 mutant strains of the bacterium Gordonia jacobaea was obtained by means of ethyl methanesulfonate treatment, and the strains were selected for their different pigmentation with respect to the wild-type strain. None of the mutants showed auxotrophy. They all showed good genetic stability and a growth rate similar to that of the parental strain. Canthaxanthin and other carotenoids from these mutants were extracted with acetone and ethanol and separated by high-performance liquid chromatography (HPLC). These HPLC analyses, together with spectrophotometric detection at 480 nm, revealed variations in the pigment contents of the different mutant strains.
通过甲磺酸乙酯处理获得了43株雅各布戈登氏菌突变菌株，并根据它们相对于野生型菌株的不同色素沉着进行了筛选。这些突变体均未表现出营养缺陷型。它们都表现出良好的遗传稳定性，生长速率与亲本菌株相似。用丙酮和乙醇从这些突变体中提取角黄素和其他类胡萝卜素，并通过高效液相色谱法（HPLC）进行分离。这些HPLC分析以及在480nm处的分光光度检测揭示了不同突变菌株色素含量的变化。
