Nakagawa H, Kiyozuka Y, Uemura Y, Senzaki H, Shikata N, Hioki K, Tsubura A
Department of Pathology II, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan.
J Cancer Res Clin Oncol. 2001 Apr;127(4):258-64. doi: 10.1007/s004320000190.
Resveratrol is a naturally occurring product found in grapes and wine. The effect of synthetic resveratrol on the growth of estrogen receptor (ER)-positive (KPL-1 and MCF-7) and -negative (MKL-F) human breast cancer cell lines was examined. Resveratrol at low concentrations caused cell proliferation in ER-positive lines (KPL-1, < or = 22 microM; MCF-7, < or = 4 microM) whereas at high concentrations (> or = 44 microM) it caused suppression of cell growth in all three cell lines examined. Growth suppression was due to apoptosis as seen by the appearance of a sub-G1 fraction. The apoptosis cascade up-regulated Bax and Bak protein, down-regulated Bcl-xL protein, and activated caspase-3. Resveratrol (52-74 microM) antagonized the effect of linoleic acid, a potent breast cancer cell stimulator, and suppressed the growth of both ER-positive and -negative cell lines. Thus, resveratrol could be a promising anticancer agent for both hormone-dependent and hormone-independent breast cancers, and may mitigate the growth stimulatory effect of linoleic acid in the Western-style diet.
白藜芦醇是一种天然存在于葡萄和葡萄酒中的产物。研究了合成白藜芦醇对雌激素受体（ER）阳性（KPL - 1和MCF - 7）及阴性（MKL - F）人乳腺癌细胞系生长的影响。低浓度的白藜芦醇会导致ER阳性细胞系（KPL - 1，≤22微摩尔；MCF - 7，≤4微摩尔）的细胞增殖，而高浓度（≥44微摩尔）时，它会抑制所检测的所有三种细胞系的细胞生长。生长抑制是由凋亡引起的，表现为亚G1期细胞比例的出现。凋亡级联反应上调了Bax和Bak蛋白，下调了Bcl - xL蛋白，并激活了caspase - 3。白藜芦醇（52 - 74微摩尔）拮抗了亚油酸（一种强效乳腺癌细胞刺激剂）的作用，并抑制了ER阳性和阴性细胞系的生长。因此，白藜芦醇对于激素依赖性和激素非依赖性乳腺癌可能都是一种有前景的抗癌药物，并且可能减轻西式饮食中亚油酸的生长刺激作用。
