Carlson B M, Dedkov E I, Borisov A B, Faulkner J A
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, 48109, USA.
J Gerontol A Biol Sci Med Sci. 2001 May;56(5):B224-33. doi: 10.1093/gerona/56.5.b224.
This study was undertaken to assess the regenerative capacity of skeletal muscle in rats near the end of their normal life span. Two experiments were performed. In the first, extensor digitorum longus (EDL) muscles were cross-age transplanted from 32-month-old male inbred Wistar (WI/HicksCar) rats in place of an EDL muscle in 4-month-old hosts. The other EDL muscle in the hosts was autotransplanted. After 60 days, the old-into-young muscle transplants regenerated as well as the young-into-young autotransplants. In the second experiment, EDL muscles in young adult (4 months) and old rats (32 and 34 months) of WI/HicksCar and Brown Norway (BN) were injected with a local anesthetic, bupivacaine, and allowed to regenerate for 41 days. In all cases, the masses and absolute maximum tetanic force of the regenerates equaled or exceeded those of untouched contralateral control muscles. These experiments showed that under appropriate conditions, very old muscles can regenerate to equal or exceed the contralateral control values, which in old rats are much less than those in muscles of young rats.
本研究旨在评估接近正常寿命末期的大鼠骨骼肌的再生能力。进行了两项实验。在第一项实验中，将32月龄雄性近交系Wistar（WI/HicksCar）大鼠的趾长伸肌（EDL）跨年龄移植到4月龄宿主的EDL肌肉位置。宿主的另一块EDL肌肉进行自体移植。60天后，老龄到幼龄的肌肉移植与幼龄到幼龄的自体移植再生情况相同。在第二项实验中，给WI/HicksCar和棕色挪威（BN）品系的成年幼龄（4个月）和老龄大鼠（32和34个月）的EDL肌肉注射局部麻醉剂布比卡因，并使其再生41天。在所有情况下，再生肌肉的质量和绝对最大强直收缩力等于或超过未处理的对侧对照肌肉。这些实验表明，在适当条件下，非常老龄的肌肉能够再生至等于或超过对侧对照值，而在老龄大鼠中，对侧对照值远低于幼龄大鼠肌肉中的值。
