Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V
Institute of Biochemistry, Medical faculty, University Clinical Center, Nis, Yugoslavia.
J Clin Pathol. 2001 May;54(5):356-61. doi: 10.1136/jcp.54.5.356.
To develop a new, simple, and cheap method for estimating antioxidant activity in human fluids.
The assay measured the capacity of the biological fluids to inhibit the production of thiobarbituric acid reactive substances (TBARS) from sodium benzoate under the influence of the free oxygen radicals derived from Fenton's reaction. A solution of 1 mmol/litre uric acid was used as standard.
The following mean (SD) antioxidative activities were found (as uric acid) in the various biological fluids: serum, 2.04 (0.20) mmol/litre; urine, 176.5 (25.6) micromol/litre; cerebrospinal fluid, 95.0 (26.9) micromol/litre; aqueous humour oculi, 61.25 (9.9) micromol/litre; saliva, 838.5 (48.2) micromol/litre; tears, 247.0 (17.0) micromol/litre; ascites fluid, 270.0 (63.3) micromol/litre; kidney cyst fluid, 387.1 (28.1) micromol/litre. Small samples of the biological material were needed for the analyses: 10 microl of serum and 50-100 microl of other body fluids. In the sera of 48 healthy individuals there was a significant positive correlation between values obtained with the Randox method (as a reference method) and the new method proposed here (correlation coefficient, 0.8728; mean difference between methods, <0.4%).
This method is easy, rapid, reliable, and practical for the routine measurement of total antioxidant activity in serum and other human body fluids. Small samples of biological material are needed for the analyses and the results are comparable with the reference (Randox) method.
开发一种用于评估人体体液中抗氧化活性的新型、简单且廉价的方法。
该检测方法测量生物体液在芬顿反应产生的游离氧自由基影响下抑制苯甲酸钠产生硫代巴比妥酸反应物质（TBARS）的能力。使用1毫摩尔/升尿酸溶液作为标准。
在各种生物体液中发现以下平均（标准差）抗氧化活性（以尿酸计）：血清，2.04（0.20）毫摩尔/升；尿液，176.5（25.6）微摩尔/升；脑脊液，95.0（26.9）微摩尔/升；眼房水，61.25（9.9）微摩尔/升；唾液，838.5（48.2）微摩尔/升；泪液，247.0（17.0）微摩尔/升；腹水，270.0（63.3）微摩尔/升；肾囊肿液，387.1（28.1）微摩尔/升。分析所需生物材料样本量小：血清10微升，其他体液50 - 100微升。在48名健康个体的血清中，用朗道克斯方法（作为参考方法）获得的值与本文提出的新方法之间存在显著正相关（相关系数，0.8728；方法间平均差异，<0.4%）。
该方法简便、快速、可靠且实用，可用于血清和其他人体体液中总抗氧化活性的常规测量。分析所需生物材料样本量小，结果与参考（朗道克斯）方法具有可比性。
