Cailhol S, Mormède P
Laboratoire de Neurogénétique et Stress, INSERM U.471-INRA-Institut François Magendie, Bordeaux Cedex, France.
Alcohol Clin Exp Res. 2001 Apr;25(4):594-9.
Alcohol drinking behavior in rats is known to be sexually dimorphic and strain-dependent.
To test whether the gonadal steroid milieu exerts activational effects on ethanol intake and can modulate individual sensitivity toward alcohol use and misuse, we examined the effects of gonadectomy on oral self-administration (OSA) of ethanol in male and female rats from different strains. After castration, animals were given continuous free choice between water and ethanol solutions. The ethanol concentration was progressively increased from 2% to 10% and maintained at 6% (the preferred concentration) for 24 days. Ethanol solutions were then withdrawn for 9 days. During the second phase of free-choice drinking, the ethanol concentration was gradually increased every 4 days by the following amounts, in order as listed: 6%, 12%, and 24%.
Our results confirm both gender and strain differences in ethanol drinking: females exhibited higher ethanol intake than males, and the WKHA strain drank more than the WKY and SHR strains. However, except for a small decrease in ethanol drinking during the acquisition of ethanol OSA in males after castration, no clear-cut difference was found between gonadectomized and sham-operated animals during the maintenance of ethanol OSA behavior.
These data suggest that gender and strain differences observed are insensitive to gonadal steroids during adulthood, and that different sensitivities to the effect of gonadal steroids do not explain the sex x strain interaction observed in ethanol drinking.
已知大鼠的饮酒行为具有性别差异和品系依赖性。
为了测试性腺类固醇环境是否对乙醇摄入产生激活作用，并能否调节个体对酒精使用和滥用的敏感性，我们研究了去势对不同品系雄性和雌性大鼠乙醇口服自我给药（OSA）的影响。去势后，给予动物在水和乙醇溶液之间持续自由选择的机会。乙醇浓度从2%逐渐增加到10%，并在6%（偏好浓度）维持24天。然后停用乙醇溶液9天。在自由选择饮酒的第二阶段，乙醇浓度每4天按以下顺序逐渐增加：6%、12%和24%。
我们的结果证实了乙醇饮用中的性别和品系差异：雌性比雄性表现出更高的乙醇摄入量，并且WKHA品系比WKY和SHR品系饮用更多。然而，除了去势后雄性大鼠在获得乙醇OSA期间乙醇饮用略有减少外，在维持乙醇OSA行为期间，去势动物和假手术动物之间未发现明显差异。
这些数据表明，观察到的性别和品系差异在成年期对性腺类固醇不敏感，并且对性腺类固醇作用的不同敏感性并不能解释在乙醇饮用中观察到的性别×品系相互作用。
