使用细菌学和血清学检测方法监测猪中肠炎沙门氏菌血清型的传播。
Monitoring of transmission of Salmonella enterica serovars in pigs using bacteriological and serological detection methods.
作者信息
van Winsen R L, van Nes A, Keuzenkamp D, Urlings H A, Lipman L J, Biesterveld S, Snijders J M, Verheijden J H, van Knapen F
机构信息
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, Utrecht, The Netherlands.
出版信息
Vet Microbiol. 2001 Jun 6;80(3):267-74. doi: 10.1016/s0378-1135(01)00313-3.
The standard method to detect Salmonella positive pigs is bacteriological examination of the faeces, but in recent years the use of Salmonella-ELISA's have become available to screen pigs for serological evidence of infection. This study was conducted to monitor the transmission of five different Salmonella enterica serovars (S. Typhimurium, S. Brandenburg, S. Panama, S. Livingstone, and S. Goldcoast) in fattening pigs and to test the feasibility of Salmonella-ELISA, using seeder pigs as a mode of transmission. Serovar dependence in transmission was observed. The Salmonella-ELISA proved to be useful to detect S. Typhimurium and S. Brandenburg in herds but was of limited value to demonstrate S. Livingstone, S. Goldcoast, and S. Panama.
检测沙门氏菌呈阳性猪的标准方法是对粪便进行细菌学检查，但近年来，沙门氏菌酶联免疫吸附测定（ELISA）已可用于筛查猪是否有感染的血清学证据。本研究旨在监测五种不同肠炎沙门氏菌血清型（鼠伤寒沙门氏菌、勃兰登堡沙门氏菌、巴拿马沙门氏菌、利文斯通沙门氏菌和黄金海岸沙门氏菌）在育肥猪中的传播情况，并以种猪作为传播途径来测试沙门氏菌ELISA的可行性。观察到传播过程中存在血清型依赖性。沙门氏菌ELISA被证明可用于检测猪群中的鼠伤寒沙门氏菌和勃兰登堡沙门氏菌，但在检测利文斯通沙门氏菌、黄金海岸沙门氏菌和巴拿马沙门氏菌方面价值有限。
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