Kamal M M, Bodele A, Munshi M M, Bobhate S K, Kher A V
Department of Pathology, Govt. Medical College, Nagpur.
Indian J Pathol Microbiol. 2000 Oct;43(4):417-21.
The chief limitation of Ultra Fast Papanicolaou (UFP) stain, suggested by Yang and Alvarez (1995), is that, Richard Allan Haematoxylin (RA-H) and Richard Allan Cytostain (RA-C), used in the staining procedure are not universally available. It has not been established so far, whether, changes in the reagents and their proportions depending on the local availability, influence the performance of the stain. The objective of this study was to assess the feasibility and applicability of a modified UFP stain to suit our local laboratory supplies of chemical dyes, as applied to the permanent smears prepared from Fine Needle Aspiration (FNA) of breast lumps. In the present study, smears from FNA from 100 breast lumps were stained by the modified UFP stain. The modification consisted of following two changes: use of Gill's Haematoxylin instead of RAH and omission of Orange G from cytostain. Eighty Six breast aspirates were adequate for interpretation. Smears showed transparent cells with crisp nuclear features, equal to and even better than the conventional Papanicolaou stain, in a blood free background. There was an increase in total staining time by 40 seconds. We recommend the use of this modified UFP stain, only if similar reagents are being used in other laboratories. Otherwise situation specific modifications may be needed. If the UFP stain is to be used for tissues where the chances of cytoplasmic keratinization are negligible, then the use of Orange G component of the stain may become redundant.
杨和阿尔瓦雷斯（1995年）指出，超快速巴氏染色法（UFP）的主要局限性在于，染色过程中使用的理查德·艾伦苏木精（RA-H）和理查德·艾伦细胞染色剂（RA-C）并非普遍可得。目前尚未确定，根据当地可得情况对试剂及其比例进行的改变是否会影响染色效果。本研究的目的是评估改良的UFP染色法应用于乳腺肿块细针穿刺（FNA）制备的永久涂片时，适用于我们当地实验室化学染料供应的可行性和适用性。在本研究中，100例乳腺肿块FNA涂片采用改良的UFP染色法进行染色。改良包括以下两点改变：使用吉尔苏木精代替RA-H，以及在细胞染色剂中省略橘黄G。86例乳腺穿刺涂片适合进行解读。涂片显示细胞透明，核特征清晰，在无血背景下与传统巴氏染色相当，甚至更好。总染色时间增加了40秒。我们建议，只有在其他实验室也使用类似试剂的情况下，才使用这种改良的UFP染色法。否则可能需要根据具体情况进行调整。如果UFP染色法用于细胞质角化可能性可忽略不计的组织，那么该染色剂中的橘黄G成分可能就多余了。
