Boonphong S, Kittakoop P, Isaka M, Palittapongarnpim P, Jaturapat A, Danwisetkanjana K, Tanticharoen M, Thebtaranonth Y
Planta Med. 2001 Apr;67(3):279-81. doi: 10.1055/s-2001-11997.
Bioassay-guided fractionation of the cell extract of the insect pathogenic fungus Aschersonia tubulata BCC 1785 led to the isolation of dustanin (1), 3 beta,15 alpha,22-trihydroxyhopane (3), 5 alpha,8 alpha-epidioxy-24(R)-methylcholesta-6,22-diene-3 beta-ol (6), together with the new 3 beta-acetoxy-15 alpha,22-dihydroxyhopane (4). Chemical structures of these compounds were elucidated by spectral analyses as well as chemical transformation. Compounds 1 and 4 exhibited antimycobacterial activity with the minimum inhibitory concentration (MIC) of 12.5 micrograms/ml.
对昆虫病原真菌管形亚隔孢壳菌Aschersonia tubulata BCC 1785的细胞提取物进行生物测定导向的分级分离，得到了达斯坦宁（1）、3β,15α,22-三羟基藿烷（3）、5α,8α-环氧-24（R）-甲基胆甾-6,22-二烯-3β-醇（6），以及新的3β-乙酰氧基-15α,22-二羟基藿烷（4）。通过光谱分析以及化学转化阐明了这些化合物的化学结构。化合物1和4表现出抗分枝杆菌活性，最低抑菌浓度（MIC）为12.5微克/毫升。
