Liberman D F, Robertson R G
Antimicrob Agents Chemother. 1975 Mar;7(3):250-5. doi: 10.1128/AAC.7.3.250.
To reduce the incubation time requirement in the Bauer-Kirby antibiotic susceptibility test, comparisons were made of the test results at 18 to 20 h (standard) and 7 to 8 h (rapid) utilizing 100 recent clinical isolates. The zone diameters for 664 disks were monitored by using the standard classification: resistant, intermediate, or susceptible. The susceptibility determination was unchanged in 558 out of 664 instances (84.0%). An analysis of the remaining 106 sets revealed that an initial interpretation of intermediate in zone size, subsequently determined resistant or susceptible, accounted for 49 of the observed differences. The reverse changes, initial resistant or susceptible subsequently classified as intermediate, accounted for 20 of the changes. In five instances the interpretation changed from susceptible to resistant; in two cases the interpretation changed from resistant to susceptible. The remaining 30 determinations were classified as indeterminant due to (i) insufficient growth at the early (7 to 8 h) determination, and to (ii) zones which were so large that they could not be measured accurately. The data indicate that zone sizes when measured to the nearest 0.1 mm can be interpreted with reasonable accuracy and the results can be available 10 to 14 h sooner.
为了缩短鲍尔-柯蒂斯抗生素敏感性试验所需的培养时间,利用100株近期临床分离株,对18至20小时(标准时间)和7至8小时(快速时间)的试验结果进行了比较。通过使用标准分类(耐药、中介或敏感)监测了664个药敏纸片的抑菌圈直径。在664例中,有558例(84.0%)的药敏判定结果未变。对其余106组结果的分析显示,最初判定抑菌圈大小为中介,随后判定为耐药或敏感的情况占观察到的差异的49例。相反的变化,即最初判定为耐药或敏感,随后归类为中介的情况占变化的20例。有5例判定结果从敏感变为耐药;有2例判定结果从耐药变为敏感。其余30次判定被归类为不确定,原因如下:(i)在早期(7至8小时)判定时生长不足,以及(ii)抑菌圈太大以至于无法准确测量。数据表明,将抑菌圈大小精确测量到最接近的0.1毫米时,可以得到合理准确的解释,并且结果可以提前十至十四小时获得。