宫颈成熟过程中宫颈黏液中透明质酸分子量及透明质酸酶活性的变化

Changes in molecular weight of hyaluronan and hyaluronidase activity in uterine cervical mucus in cervical ripening.

作者信息

Obara M, Hirano H, Ogawa M, Tsubaki H, Hosoya N, Yoshida Y, Miyauchi S, Tanaka T

机构信息

Department of Obstetrics and Gynecology, Akita University School of Medicine, Akita, Japan.

出版信息

Acta Obstet Gynecol Scand. 2001 Jun;80(6):492-6.

PMID:11380283

Abstract

BACKGROUND

This study was undertaken to investigate changes in the forms of hyaluronan and hyaluronidase activity in cervical mucus during cervical ripening.

METHODS

Uterine cervical mucus was obtained from 57 pregnant women (25 at preterm gestation, ten at term gestation, 11 within 1 week before labor, and 11 during the first stage of labor). We determined 1) concentration of hyaluronan, 2) hyaluronidase activity, and 3) molecular weight of hyaluronan in cervical mucus. Data are presented as mean and range.

RESULTS

The hyaluronan concentration in mucus in the 1st stage of labor (1.58 microg/ml, 0.46-23.96) was significantly (p<0.05) higher than that in all other groups (preterm: 0.29, 0.10-0.88; term: 0.24, 0.11-0.80; within 1 week before labor: 0.30, 0.18-0.62). Hyaluronidase activity both within 1 week before labor group (3.03 min., 1.12-3.95) and in 1st stage of labor group (3.52, 0.43-5.15) was significantly (p<0.05) higher than that in preterm group (1.70, 0.00-5.47). The molecular weight of hyaluronan in cervical mucus in the 1st stage of labor (0.97x106, 0.86-1.41) was significantly (p<0.05) lower than in the preterm and term groups (preterm: 1.60, 1.21-2.20, term: 1.41, 1.21-2.20). There was a significant correlation between hyaluronidase activity and molecular weight of hyaluronan (p<0.05, r=-0.41, n=23).

CONCLUSION

These findings suggest that either hyaluronidase or low-molecular weight hyaluronan could be one of the most important regulators in the process of cervical ripening.

摘要

背景

本研究旨在调查宫颈成熟过程中宫颈黏液中透明质酸形式和透明质酸酶活性的变化。

方法

从57名孕妇获取宫颈黏液（25名早产孕妇、10名足月孕妇、11名临产前1周内孕妇以及11名第一产程孕妇）。我们测定了1）宫颈黏液中透明质酸的浓度、2）透明质酸酶活性以及3）透明质酸的分子量。数据以均值和范围表示。

结果

第一产程时黏液中的透明质酸浓度（1.58微克/毫升，0.46 - 23.96）显著高于所有其他组（早产组：0.29，0.10 - 0.88；足月组：0.24，0.11 - 0.80；临产前1周内组：0.30，0.18 - 0.62）（p<0.05）。临产前1周内组（3.03分钟，1.12 - 3.95）和第一产程组（3.52，0.43 - 5.15）的透明质酸酶活性均显著高于早产组（1.70，0.00 - 5.47）（p<0.05）。第一产程时宫颈黏液中透明质酸的分子量（0.97×10^6，0.86 - 1.41）显著低于早产组和足月组（早产组：1.60，1.21 - 2.20；足月组：1.41，1.21 - 2.20）（p<0.05）。透明质酸酶活性与透明质酸分子量之间存在显著相关性（p<0.05，r = -0.41，n = 23）。