Le Marchand L, Lum-Jones A, Saltzman B, Visaya V, Nomura A M, Kolonel L N
Etiology Program, Cancer Research Center of Hawaii, University of Hawaii, Honolulu, Hawaii 96813, USA.
Cancer Epidemiol Biomarkers Prev. 2001 Jun;10(6):701-3.
This study assessed the feasibility of obtaining buccal cell DNA by mail from participants in a large, community-based cohort study in Hawaii. Mouthwash collection kits were sent to a total of 355 randomly selected Japanese, Caucasian, and Hawaiian cohort members. Subjects were requested to swish 10 ml of mouthwash in their mouth for 60 s and expel it into a collection cup, which they mailed back to our laboratory. Half of the subjects were requested to collect a second sample. After up to two mailings and two reminder phone calls, two-thirds of the subjects returned a sample. The participation rate was lower for Hawaiians (59.0%) than for Caucasians (68.1%) and Japanese (76.3%). Participation was not affected by requesting two specimens. Participants did not differ from the total sample in terms of education and smoking status. The mean DNA yield was lower in females (41.7 microg) than males (53.4 microg) and in Japanese (37.8 microg) as compared with Hawaiians (51.9 microg) and Caucasians (54.5 microg). For subjects who returned two samples, the DNA yields were similar when both specimens were extracted in the same batch. All samples were successfully genotyped for polymorphisms in the CYP1A1, CYP2E1, GSTM1, GSTT1, and NQO1 genes by PCR-RFLP. From these and previous data, we conclude that, in situations where blood samples cannot be obtained, mail collection of mouthwash samples should be considered because it yields substantial amounts of high-quality genomic DNA for large numbers of study subjects.
本研究评估了在夏威夷一项大型社区队列研究中，通过邮件从参与者处获取颊细胞DNA的可行性。漱口水收集试剂盒被寄给了总共355名随机挑选的日本、白种人和夏威夷队列成员。要求受试者用10毫升漱口水在口腔中漱口60秒，然后将其吐入收集杯中，再寄回我们的实验室。一半的受试者被要求收集第二个样本。经过最多两次邮寄和两次提醒电话后，三分之二的受试者寄回了样本。夏威夷人的参与率（59.0%）低于白种人（68.1%）和日本人（76.3%）。要求采集两份样本并未影响参与情况。参与者在教育程度和吸烟状况方面与总样本没有差异。女性（41.7微克）的平均DNA产量低于男性（53.4微克），日本人（37.8微克）的平均DNA产量低于夏威夷人（51.9微克）和白种人（54.5微克）。对于寄回两份样本的受试者，当两份样本在同一批次提取时，DNA产量相似。通过PCR-RFLP对所有样本成功进行了CYP1A1、CYP2E1、GSTM1、GSTT1和NQO1基因多态性的基因分型。根据这些数据和之前的数据，我们得出结论，在无法获取血样的情况下，应考虑通过邮件收集漱口水样本，因为它能为大量研究对象提供大量高质量的基因组DNA。
