Narvel J M, Jakkula L R, Phillips D V, Wang T, Lee S H, Boerma H R
Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602-7272, USA.
J Hered. 2001 May-Jun;92(3):267-70. doi: 10.1093/jhered/92.3.267.
The Rxp locus in soybean [Glycine max (L.) Merr.] that conditions reaction to bacterial pustule was mapped by simple sequence repeat (SSR) marker analysis. A population of 116 F4-derived lines from a cross between the resistant parent Young and the susceptible parent PI 416937 was used for mapping. The Rxp locus was mapped 3.9 cM from Satt372 and 12.4 cM from Satt014 on linkage group D2. Linkage associations were confirmed by identifying a close association between the SSR genotype at each locus identified as flanking Rxp and the bacterial pustule reaction of individual lines derived from a population different from the one used for mapping. A molecular pedigree analysis showed that bacterial pustule-resistant cultivars inherited the resistance gene rxp from the ancestral cultivar CNS based on their consistent genotypic pattern at flanking marker loci. Based on the results of the study, marker-assisted selection for rxp would be very effective.
通过简单序列重复（SSR）标记分析对大豆[Glycine max (L.) Merr.]中决定细菌性斑点病抗性反应的Rxp位点进行了定位。利用抗性亲本Young与感病亲本PI 416937杂交得到的116个F4衍生株系群体进行定位。Rxp位点在连锁群D2上距离Satt372为3.9厘摩，距离Satt014为12.4厘摩。通过鉴定被确定为位于Rxp两侧的每个位点的SSR基因型与来自不同于用于定位的群体的各个株系的细菌性斑点病反应之间的紧密关联，证实了连锁关系。分子谱系分析表明，基于细菌性斑点病抗性品种在侧翼标记位点的一致基因型模式，它们从祖先品种CNS继承了抗性基因rxp。基于该研究结果，对rxp进行标记辅助选择将非常有效。
